This study aimed to investigate the effects of epidermal growth factor (EGF) on the proliferation and differentiation of adipose stem cells (ASC) during the repeated passaging and probe the underlying signal pathway. Results showed that the Ki67 positive rate remained at a high level, the number of ASCs in G0/G1 phase reduced significantly, but ASCs in G2/M phase and S phase increased markedly ...

To determine the role of epidermal growth factor (EGF)-stimulated protein kinase in the biological effects caused by EGF, tyrosine-specific kinase activity has been quantitated in A431 human epidermoid carcinoma cells and six variant cell lines. Because EGF inhibited proliferation of A431 cells, variants resistant to this inhibition were selected by treatment with mutagen and maintenance for 1 ...

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) has been shown to stimulate the growth of a variety of cells in an autocrine or paracrine manner. Although HB-EGF is widely expressed in tumors compared with normal tissue, its contribution to tumorigenicity is unknown. HB-EGF can be produced as a membrane-anchored form (pro-HB-EGF) and later processed to a soluble form (s-HB-E...

signaling pathways are not isolated from their surroundings. they are also intervened by other signaling pathways known as “crosstalk mechanism”. one of the most important crosstalk mechanisms is the insulinegf network. although insulin and epidermal growth factor (egf) networks have some complexity in their isolated forms, their complexities will grow in the crosstalk network. in this study, w...

background : st6gal1, cox-2 and hb-egf genes are involved in different tumors and their enhanced expressions often correlate with poor prognosis. in this study we assay the expressions of these genes by reverse transcriptase-pcr in 54 breast cancer samples. methods : tissue samples were either formalin-fixed for histopathological examination or frozen for reverse transcriptase-pcr. image progra...

Epidermal growth factor (EGF) may play a role in regulating growth of breast cancer cells in vivo. We have examined the action of EGF on breast cancer cells in vitro and characterized the EGF receptor as a model system for its action in vivo. All of the fourteen breast cancer cell lines which grow attached to culture dishes specifically bound EGF, including one purportedly normal breast line (H...

Epidermal growth factor (EGF) along with several related peptide growth factors has been shown both in vivo and in vitro to accelerate events associated with epidermal wound repair. EGF and transforming growth factor alpha act by binding to a common EGF receptor tyrosine kinase thereby initiating a series of events which ultimately regulate cell proliferation. This study examined the immunohist...

Maréchal, Hervé, Hélene Jammes, Bernard Rossignol, and Philippe Mauduit. EGF precursor mRNA and membrane-associated EGF precursor protein in rat exorbital lacrimal gland. Am. J. Physiol. 276 (Cell Physiol. 45): C734– C746, 1999.—This study was designed to demonstrate the presence of epidermal growth factor (EGF) in the rat exorbital lacrimal gland. EGF precursor gene transcription was demonstra...

Abstract Background Vacuum sealing drainage (VSD) and epidermal growth factor (EGF) both play an important role in the treatment of wounds. This study aims to explore effects combination VSD EGF on wound healing optimal concentration time EGF. Methods We tested proliferation migration capacity HaCaT L929 cells at different concentrations (0, 1, 5, 10, 100 ng/ml) action times (2, 30 min). A full...

Retinoids are potent regulators of epithelial cell growth and differentiation. Recently, they have been demonstrated to be effective in the treatment of preneoplastic cervical lesions in which human papillomavirus (HPV) is expressed. To better understand the mechanism of the antineoplastic effect of retinoic acid on HPV-positive cells, the effects of retinoic acid on both normal and HPV-immorta...