A pregnancy at risk for the Sanfilippo syndrome has been studied in which clear evidence was obtained from the study of amniotic fluid and fetal organs that the fetus was affected. Increased levels ofheparan sulphate were found in amniotic fluid and fetal liver, while electronmicroscopy of cultured fetal fibroblasts and fetal liver showed abnormal cytoplasmic inclusions. 35S04 uptake studies of...

The malignant cells in a patient with hairy cell leukemia responded most evidently to lipopolysaccharide (LPS) in in vitro culture for 3 1/2 days when the conventional tritiated thymidine uptake method was used. Since the malignant cells from patients with several other forms of leukemia and the peripheral blood mononuclear cells from healthy individuals did not show a comparable degree of resp...

A case of systemic lupus erythematosus is described which clinically resembled multiple sclerosis and in which the lesions were restricted to the central nervous system. The necropsy findings of vascular thickening and necrosis in the spinal cord and in a posterior nerve root explain the main clinical abnormalities. Clinical signs of the terminal peritonitis secondary to cholecystitis were abse...

The appearance of M. lepraemurium as seen by electronmicro­ scopy has been described by McFadzean and Valentine (1958-9). Two forms of the bacilli were observed, one the normal form (Fig. 1 ) and the other a degenerate non-viable form (Fig . 2). It was shown that an estimate of the percentage of degenerate forms present gave an indication as to the viability of a suspension. This paper describe...

Forssman antigen, a neutral glycosphingolipid carrying five monosaccharides, was localized in epithelial MDCK cells by the immunogold technique. Labeling with a well defined mAb and protein A-gold after freeze-substitution and low temperature embedding in Lowicryl HM20 of aldehyde-fixed and cryoprotected cells, resulted in high levels of specific labeling and excellent retention of cellular ult...

Two antigens of Taenia solium cysticercus, cystic fluid antigen (CFA) and the culture medium antigen (CMA), were used respectively to immunize rabbits in order to obtain immunosera. The CMA immunoserum added to culture medium with cysticerci limited the activities of the bladder worms. By using the scanning electronmicroscope, we could observe particulate deposits on the surface of the scolices...

A case of Bowen's disease arising on the medial part of the first metatarsal bone of an 81-year-old Japanese woman is described. Histopathologically, proliferation of atypical cells was found throughout the epidermis. Electronmicroscopy revealed virus particles 40-50 nm in diameter in the nuclei of tumour cells at the granular cells just on or below the horny layer. Positive bands were obtained...

The authors have analyzed clinico-neuropathologically nine cases of the definite sporadic form of Creutzfeldt-Jakob disease (CJD). All cases were female, with mean age of 62.7 years. Eighty-nine percent of the patients exhibited prodromal and initial psychiatric symptoms; definite signs of dementia, and myoclonus were present in 100% of cases. The EEG was abnormal in all cases and pseudoperiodi...

It was demonstrated earlier that the renal venous effluent of one-kidney, one clip hypertensive rats contained a vasodepressor lipid resembling the antihypertensive neutral renomedullary lipid (ANRL), following unclipping and as the arterial pressure (MAP) was lowered. Consequently, the sham-unclipped (clip-intact) and the unclipped kidney (CK and UCK) were studied by electronmicroscopy and mo...

By G. F. SMITH, HELGA KOLB AND JANET A. FAur’ cii DURING AN ELECTRON-MICROSCOPIC INVESTIGATION of cultured human lymphocytes, an intercellular or spindle-bridge and mid-body were observed (Figs. 1 and 2). These unusual structures occur in late telophase of cell division. While they have been demonstrated by electronmicroscopy in mammalian secondary spermatocytes,’ cnidoblasts of Hydra,2.3 rat e...