OBJECTIVE To prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis and develop double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) for the detection of genital C. trachomatis infections. METHODS The pORF5 protein was expressed in Escherichia coli and used to immunize BALB/c mice and New Zealand rabbits to produce monoclonal antibodies (mAbs) and polycl...

background : toxoplasmosis is a worldwide endemic disease. in congenitally infected infants and aids patients, toxoplasmosis causes high rates of morbidity and mortality. in these cases antibody detection is difficult; so detection of parasite or its components could be useful tool for early detection and following treatment of the infection. methods : sixty-three balb/c mice were injected intr...

DAS-ELISA, immunohistochemistry and electron microscopy were used to investigate the association of the causal agent of bacterial wilt, Erwinia tracheiphila (Smith), within the beetle Acalymma vittatum (F.). After a 24-h acquisition period, a high percentage of individuals tested positive for E. tracheiphila antigen using both immunohistochemistry (100%) and DAS-ELISA (70-60%). Both assays show...

The presence of grapevine virus B (GVB) was detected in 188 grapevine samples from China by double antibody sandwich ELISA (DAS-ELISA) and reverse transcription-PCR (RT-PCR). The accuracy of detection by RT-PCR was confirmed by sequencing amplified PCR fragments. Seventeen samples were GVB-positive by DAS-ELISA and five by RT-PCR. The isolate QMW proved to be positive by RT-PCR only, and four i...

abstract during the winter and spring of 2007 and 2008, 450 samples (leaves of carnation plants) showing symptoms of yellowing, mottling, leaf malformation, and stunting were collected from greenhouses in mashhad and chenaran regions. the samples were tested by das-elisa for the presence of carmv. total rna was extracted by rnxtm (-plus) solution from positive infected samples confirmed in das-...