Background and Aims. Human embryonic stem cell- (hESC-) derived cardiomyocytes are one of the useful screening platforms of potential cardiocytoprotective molecules. However, little is known about the behavior of these cardiomyocytes in simulated ischemia/reperfusion conditions. In this study, we have tested the cytoprotective effect of an NO donor and the brain type natriuretic peptide (BNP) i...

introduction: stem cell biology has been the subject of much recent discussion. embryonic stem (es) cells, derived from the inner cell mass of the blastocyst stage of early mammalian embryos are expected to become a powerful tool in future regenerative medicine and developmental biology due to their capacity of self-renewal and pluripotency. in the present study, the ultrastructural development...

the use of quantum dots (qdots) as bright and photostable probes for long-term fluorescence imaging is gaining more interest. thus far, (pre)clinical use of qdots remains limited, which is primarily caused by the potential toxicity of qdots. most qdots consist of cd2+ ions, which are known to cause high levels of toxicity. therefore, the cytotoxic effects of cdse quantum dots on embryonic devel...

Cancer tissues have biological characteristics similar to those observed in embryos during development. Many types of cancer cells acquire pro-invasive ability through epithelial-mesenchymal transition (EMT). Similar processes (gastrulation and migration of cranial neural crest cells [CNCC]) are observed in the early stages of embryonic development in Xenopus during which cells that originate f...

There is an interesting overlap of function in a wide range of organisms between genes that modulate the stress responses and those that regulate aging phenotypes and, in some cases, lifespan. We have therefore screened mutagenized zebrafish embryos for the altered expression of a stress biomarker, senescence-associated beta-galactosidase (SA-beta-gal) in our current study. We validated the use...

ABSTRACT In mammals, sperm-borne regulators can be transferred to oocytes during fertilization and have different effects on the formation of pronuclei, first cleavage zygotes, development preimplantation embryos even metabolism individuals after birth. The regulatory role sperm microRNAs (miRNAs) in bovine has not been reported detail. By constructing screening miRNA expression libraries, we f...

BACKGROUND Preimplantation genetic screening (PGS) has been used in an attempt to determine embryonic aneuploidy. Techniques that use new molecular methods to determine the karyotype of an embryo are expanding the scope of PGS. METHODS We introduce a new method for PGS, termed 'parental support', which leverages microarray measurements from parental DNA to 'clean' single-cell microarray measu...

In this review, we evaluate the different modalities of embryo genetic testing including preimplantation for aneuploidy (PGT-A), monogenic/single-gene abnormalities (PGT-M), and chromosomal structural rearrangements (PGT-SR), with a clinical focus on indications, strengths, limitations, parameters each technique. Articles were obtained from PubMed American College Obstetricians Gynecologists So...