INTRODUCTION Gonorrhoea is a common sexually transmitted disease caused by Neisseria gonorrhoeae (Ng) infection. Light microscopy of urogenital smears is used as a simple tool to diagnose urogenital gonorrhoea in many resource-limited settings. We aimed to evaluate the accuracy of light microscopy to diagnose urogenital gonorrhoea as compared with a PCR-based test. METHODS In 2014, we examine...

The aim of this study was to determine whether a patient's endocervical swab specimen can be transported in first void urine (FVU) as combined specimens for the detection of Mycoplasma genitalium by real-time PCR. The study also compared two different DNA extraction methods for observation of possible PCR inhibition. Three specimens, one endocervical swab specimen transported in 2-SP medium, on...

I read with interest the article by Ozkan and colleagues1 on glandular lesions of the cervix on thinlayer Pap tests. However, a few clarifications need to be made. The authors commented on the difficulty distinguishing endocervical adenocarcinoma in situ (AIS) from high grade squamous intraepithelial lesion (HSIL) involving endocervical glands. Of the 37 ThinPrep® Pap TestsTM (Cytyc Corporation...

Endocervical swabs from 212 women and urethral swabs from 100 men were tested by the routine methods for McCoy cell culture and simultaneously by a novel enzyme amplified immunoassay test to detect chlamydia antigen. Overall correlation of the amplified test with culture was 96.5%. The test proved to be a suitable screening procedure for genital chlamydial infection, particularly for large numb...

OBJECTIVE To evaluate the use of liquid cytology in Pap smears in women with developmental disabilities (DD) for endocervical cell yield and abnormalities, via speculum examination or blind technique. METHODS We used retrospective chart review of gynecological visits by women with DD from October 2002 to November 2005. Cervical cytology screening included speculum examination or blind techniq...

chlamydia trachomatis infections are the most prevalent bacterial sexually transmitted infections (sti) recognized throughout the world. the aim of this study was to determine the prevalence of chlamydia trachomatis among a randomized statistical group of women suffering from cervicitis in tehran- iran. during a 12- month- period, jan 2003 to jan 2004, 142 endocervical samples were taken from w...

Mucosal epithelia of the human lower reproductive tract (vagina, cervix, and penile urethra) are exposed to sexually transmitted microbes, including Chlamydia trachomatis. The in vivo susceptibility of each tissue type to infection with C. trachomatis is quite distinct. CD1d is expressed on the surface of antigen-presenting cells, including mucosal epithelial cells, and interacts specifically w...