Cryo-electron-microscopy (cryo-EM) structures of flaviviruses reveal significant variation in epitope occupancy across different monoclonal antibodies that have largely been attributed to epitope-level differences in conformation or accessibility that affect antibody binding. The consequences of these variations for macroscopic properties such as antibody binding and neutralization are the resu...

BUMC PROCEEDINGS 2002;15:16–17 Celiac sprue, a gluten-induced enteropathy, was once considered primarily an immunologic disease of infants and children. Gluten is a protein found in grains, primarily wheat and barley. The ingestion of gliadin, a component of gluten, causes gastrointestinal symptoms including malabsorption and multiple nutritional deficiencies. Our understanding of this disorder...

Background & Aim: The recent study was conducted to investigate the effects of Thyme extract in drinking water on immune response of broiler chickens. Experimental:  A total of 245 day-old broiler chicks were purchased and 20 chicks were bled for determination maternal antibody and remaining chicks divided into 5 equal groups. Chickens of group A, B and C received 0.1%, 0.15% and 0.2%...

Background & Aim: The recent study was conducted to investigate the effects of Thyme extract in drinking water on immune response of broiler chickens. Experimental:  A total of 245 day-old broiler chicks were purchased and 20 chicks were bled for determination maternal antibody and remaining chicks divided into 5 equal groups. Chickens of group A, B and C received 0.1%, 0.15% and 0.2%...

Postembedding immunolabeling using resin sections is the recommended method for beginners carrying out electron microscopy (EM) immunolabeling. Postembedding labeling refers to labeling on sections, which is a method of gaining access to the interior of the cell without the harshness of detergent or ionic extraction as is performed with preembed labeling. Investigators already familiar with rou...

Standard methods of virus diagnosis may take many days to complete. As antiviral drugs are being used with more effectiveness, it becomes more important to develop rapid diagnostic methods. It takes only a few minutes to prepare and examine a specimen for electron microscopy (EM), using the negative staining technique. Viruses in the specimen can readily be identified by their morphology. In or...

The diagnostic performance of an enzyme-linked immunosorbent assay (ELISA) using purified Borrelia burgdorferi flagella as test antigen was compared with that of a B. burgdorferi sonic extract ELISA. We tested sera from 200 healthy controls, 107 patients with erythema migrans (EM), 50 patients with acrodermatitis chronica atrophicans (ACA), and 98 patients with various dermatological disorders ...

Background: Celiac disease (CD) is an autoimmune affecting the small intestine, triggered by gluten. The pathogenesis of CD has been well defined, and increasing prevalence this had led to studying several environmental risk factors that may trigger process in intestine. Infection one most important can processes. researchers have studied association between H. pylori infection CD, authors repo...

Background and Aim: Celiac Disease (CeD) is a multisystemic disease in addition to the small intestine, also affects liver. The spectrum of liver involvement CeD includes cryptogenic hypertransaminasaemia/cirrhosis, autoimmune diseases vascular like Non-cirrhotic portal fibrosis (NCPF). There lack systematic study on prevalence patients with NCPF. Methods: All new biopsy-proven NCPF reporting c...

Myocardial connective tissue probably provides passive support for regulating heart tensile strength and stiffness and ultimately for controlling heart mechanics through its endomysial part. However, endomysial collagen micro-arrangement is still a matter of debate. In order to define the fine distribution of left ventricle endomysial collagen, we applied the NaOH-scanning electron microscopy (...