introduction: the aim of this study was to compare the antimicrobial effects of 2.5% sodium hypochlorite (naocl), 2% chlorhexidine gluconate (chx) and biopure mtad (mtad) on enterococcus (e) faecalis -contaminated root canals of human extracted teeth. materials and methods: seventy human intact extracted single-rooted teeth with straight root canal randomly divided into 5 groups: positive contr...

conclusions according to these findings, antibiotic-resistance patterns have changed, and vancomycin resistance, especially among e. faecium, is rising because of nosocomial infections. consequently, it has become a serious subject for patients admitted into a hospital. results a total of 53 enterococci were isolated from clinical samples of blood, urine, wounds, sputum, and cerebrospinal fluid...

Most Enterococcus faecalis and E. faecium are harmless to humans; however, strains harboring virulence genes, including esp, gelE, cylA, asa1, and hyl, have been associated with human infections. E. faecalis and E. faecium are present in beach waters worldwide, yet little is known about their virulence potential. Here, multiplex PCR was used to compare the distribution of virulence genes among ...

The presence, distribution and expression of cassette chromosome recombinase (ccr) genes, which are homologous to the staphylococcal ccrAB genes and are designated ccrAB(Ent) genes, were examined in enterococcal isolates (n=421) representing 13 different species. A total of 118 (28 %) isolates were positive for ccrAB(Ent) genes by PCR, and a number of these were confirmed by Southern hybridizat...

Enterococci are members of the lactic acid bacteria family and are responsible for many food spoilage and fermentations. Some strains of this microorganism are used as probiotics in humans and animals to improve host immunity. However, some Enterococci are important pathogens which cause severe infections. Some strains of Enterococci are resistant to common antibiotic...

House flies are among the most important nonbiting insect pests of medical and veterinary importance. Larvae develop in decaying organic substrates and their survival strictly depends on an active microbial community. House flies have been implicated in the ecology and transmission of enterococci, including multi-antibiotic-resistant and virulent strains of Enterococcus faecalis. In this study,...

As a reference laboratory, the Streptococcus Laboratory at the Centers for Disease Control and Prevention (CDC) is frequently asked to confirm the identity of unusual or difficult-to-identify catalase-negative, gram-positive cocci. In order to accomplish the precise identification of these microorganisms, we have systematically applied analysis of whole-cell protein profiles (WCPP) and DNA-DNA ...

background: to determine the species distribution, updated drug susceptibility patterns and genes conferring resistance in clini­cal vancomycin resistant enterococcal (vre) isolates. methods: clinical enterococcal isolates collected during 7 months, from september 2005 to april 2006 from hos­pitalized pa­tients and outpatients were studied. twenty five vre were isolated from 450 enterococci sam...

The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999–2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent gramnegatives and grampositives, the common part of intestinal microflora and also...

A total of 12 VanA-type vancomycin-resistant enterococci, consisting of 10 Enterococcus faecium isolates and two Enterococcus avium isolates, were examined in detail. The vancomycin resistance conjugative plasmids pHTalpha (65.9 kbp), pHTbeta (63.7 kbp), and pHTgamma (66.5 kbp) were isolated from each of three different E. faecium strains. The plasmids transferred highly efficiently between ent...