diarrheal diseases can be caused by viral, bacterial and parasitic infections. this paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in tunisian children less than five years of age.overall, 124 diarrhoeal stools were collected from patients suffering from acute diarrhea and 54 stool samples from healthy children. all stools were examined for the...

background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...

Abstract Enteropathogenic E. coli (EPEC) causes intestinal infections leading to severe diarrhea. EPEC attaches the host cell causing lesions epithelium coupled with effacement of microvilli. In process, actin accumulates into a pedestal-like structure under bacterial microcolonies. We designed an automated fluorescence microscopy-based screening method for discovering compounds capable inhibit...

Abstract Background Diarrhoea is a major cause of childhood disease in the developing countries. This experimental study investigated prevalence ESBL and MBL genes enteropathogenic strains Escherichia coli Salmonella spp. isolated from diarrheagenic children Awka, Nigeria. Methods Two hundred stool samples were collected diarrhea patients three paediatric hospitals within Awka metropolis, All E...