نتایج جستجو برای: enzyme immobilization
تعداد نتایج: 254179 فیلتر نتایج به سال:
Industrial applications require enzymes highly stable and economically viable in terms of reusability. Enzyme immobilization is an exciting alternative to improve the stability of enzymatic processes. The immobilization of two commercial enzymes is reported here (cellulase and xylanase) using three chemical methods (adsorption, reticulation, and crosslinking-adsorption) and two polymeric suppor...
Immobilization of enzymes is interesting research topic from both fundamental and applied aspects, and various kinds of immobilization methods including chemical and physical procedures have been actively investigated, e.g. the chemical conjugation of enzyme with water-soluble polymers and the physical entrapment into polymer gel matrix (1-5). The advantages of enzyme-immobilization were improv...
This paper describes the immobilization of the neutral protease from Bacillus subtilis and its application in the regioselective hydrolysis of acetylated nucleosides, including building blocks useful for the preparation of anticancer products. Regarding the immobilization study, different results have been obtained depending on the immobilization procedure. Epoxy hydrophobic carriers gave a poo...
The immobilization of cellulases could be an economical alternative for cost reduction of enzyme application. The derivatives obtained in the immobilization derivatives were evaluated in recycles of paper filter hydrolysis. The immobilization process showed that the enzyme recycles were influenced by the shape (drop or sheet) and type of the mixture. The enzyme was recycled 28 times for sheets ...
This paper describes the impact of a physical immobilization methodology, using plasma polymerized 1,1,3,3, tetramethyldisiloxane, on the catalytic performance of β-galactosidase from Aspergillus oryzae in a microfluidic device. The β-galactosidase was immobilized by a polymer coating grown by Plasma Enhanced Chemical Vapor Deposition (PEVCD). Combined with a microchannel patterned in the silic...
Objective: B.subtilis and B.licheniformis have been reported as one of the potential protease producer. Hence, an attempt has been made to optimize and compare the enzyme production by entrapment and fermentor conditions. Methods: Growth profile of both the strains has been studied. Optimization of culture conditions with respect to pH, temperature, agitation, carbon, nitrogen sources and metal...
The current demands of the world's biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. These requirements are inevitable to facilitate large-scale and economic formulation. Enzyme immobilization provides an excellent base for increasing availability of enzyme to the substrate with greater turnover over a conside...
Enzyme immobilization is a specific method for restricting the enzyme freedom of movement. There are strategies for the proteins multipoint immobilization by amine-terminal residues, lysine residues and carboxylic groups. In the present work, a commercial invertase of Saccharomyces cerevisiae underwent multipoint immobilization on glyoxyl-agarose, amineagarose and glutaraldehyde-agarose support...
Sodium chloride salting-in and microwave irradiation were combined to drive thermolysin molecules into mesoporous support to obtain efficiently immobilized enzyme. When the concentration of sodium chloride was 3 M and microwave power was 40 W, 93.2% of the enzyme was coupled to the support by 3 min, and the maximum specific activity of the immobilized enzyme was 17,925.1 U mg(-1). This was a 4....
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