A model of Mg metabolism in sheep is proposed. It is based on standard Michaelis-Menten enzyme kinetics to describe the transport of Mg across the rumen wall and passive diffusion to describe the absorption of Mg in the hindgut. Factors known to have an effect on Mg metabolism in farm animals, namely the concentrations of K and Mg in the diet, and the physico-chemical conditions within the rume...

Highly efficient and rapid lead compound evaluation for estimation of inhibition parameters and type of inhibition is proposed. This is based on a single progress-curve measurement in the presence of each candidate compound, followed by the simultaneous analysis of all of these curves using the ENZO enzyme kinetics suite, which can be implemented as a web application. In the first step, all of ...

A method is described for fitting equations to enzyme kinetic data that requires minimal assumptions about the error structure of the data. The dependence of the variances on the velocities is not assumed, but is deduced from internal evidence in the data. The effect of very bad observations ('outliers') is mitigated by decreasing the weight of observations that give large deviations from the f...

The temporal autocorrelation (AC) function associated with monitoring order parameters characterizing conformational fluctuations of an enzyme is analyzed using a collection of surrogate models. The surrogates considered are phenomenological stochastic differential equation (SDE) models. It is demonstrated how an ensemble of such surrogate models, each surrogate being calibrated from a single t...

The principle of enzyme kinetics suggests that the temperature sensitivity (Q10) of soil organic matter (SOM) decomposition is inversely related to organic carbon (C) quality, i.e., the C quality-temperature (CQT) hypothesis. We tested this hypothesis by performing laboratory incubation experiments with bulk soil, macroaggregates (MA, 250-2000 μm), microaggregates (MI, 53-250 μm), and mineral f...

Tihonov's Theorems for systems of first-order ordinary differential equations containing small parameters in the derivatives, which form the mathematical foundation of the steady-state approximation, are restated. A general procedure for simplifying chemical and enzyme reaction kinetics, based on the difference of characteristic time scales, Is presented. Korzuhin's Theorem, which makes it poss...

Lastly, I wish to illustrate and to stress the necessity of assessing goodness of fit by making use of several tests and not relying on only one or two such tests. Referring to Table 1, the xz test and the Runs test indicate that model 3 is a good fit, whereas the curved Rankits plot, the slow convergence, the non-linearity at the minimum sum of squares and the wide range of the coefficients of...