human erythrocyte transketolase (sedoheptulose-7-phosphate: d-glyceraldehyde-3-phosphate, glycolaldehyde transferase, e.c. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 u/mg. sds-page and se-hplc were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. four protein bands corresponding to molecular weights of 32,000, 3...

We investigated the mechanism by which the three most commonly measured enzymes in erythrocytes are activated by their respective coenzymes by determining the catalytic activity concentrations of transketolase (EC 2.2.1.1), aspartate aminotransferase (EC 2.6.1.1), and glutathione reductase (EC 1.6.4.2) in relation to various substrate concentrations. We conclude that the underlying mechanisms b...

CorreSpondence Measurement of erythrocyte transketolase activity Correspondence from Donald E. King C. R. Milner et al.'s recent paper compared automated versus manual procedures for the 'Measurement of erythrocyte trans-ketolase activity on a discrete analyser' (Journal of Automatic Chemistry, Vol. 4, No. 4, pp. 183-185). In it the author uses regression analysis to evaluate the similarity of ...

The conversion of hexose monophosphate to sedoheptulose 7phosphate, first detected by Bonsignore et al. (1) in dialyzed liver extracts, and by Dische (2) in red cell hemolysates, can be attributed to the presence of transketolase and transaldolase in these extracts (3, 4). With highly purified preparations of transketolase and transaldolase, free of phosphoglucose isomerase and xylulose 5-phosp...

The conversion of hexose monophosphate to sedoheptulose 7phosphate, first detected by Bonsignore et al. (1) in dialyzed liver extracts, and by Dische (2) in red cell hemolysates, can be attributed to the presence of transketolase and transaldolase in these extracts (3, 4). With highly purified preparations of transketolase and transaldolase, free of phosphoglucose isomerase and xylulose 5-phosp...

Amycolatopsis mediterranei produces an important antibiotic rifamycin, the biosynthesis of which involves many unusual modifications. Previous work suggested a putative P450 enzyme encoded by rif16 within the rifamycin biosynthetic gene cluster (rif) was required for the conversion of the intermediate rifamycin SV into the end product rifamycin B. In this study, we genetically proved that a put...

The conversion of hexose monophosphate to sedoheptulose 7phosphate, first detected by Bonsignore et al. (1) in dialyzed liver extracts, and by Dische (2) in red cell hemolysates, can be attributed to the presence of transketolase and transaldolase in these extracts (3, 4). With highly purified preparations of transketolase and transaldolase, free of phosphoglucose isomerase and xylulose 5-phosp...