Kinetic methods for the quantification of substrates offer a number of advantages over equilibrium methods, such as speed and economy. However, in their most commonly practiced form they are liable to error from various sources in addition to those ordinarily encountered in equilibrium methods, owing to variations in enzymic activity between test and standard assays. In certain circumstances th...

azobenzene for 60 days. The muscle type aldolase in the livers of rats thus treated was found to be elevated when compared to nor mal liver, although no appreciable differences were ob served in total aldolase activity, mitotic index, histological architecture, and soluble protein, as well as DNA content. The appearance of this enzymic change was already observable by administrating the azo dye...

The proteolysis of bovine pancreatic ribonuclease by subtilisin has been shown to involve the formation of an enzymically active intermediate which is further degraded to inactive products (1, 2). This paper describes the preparation of the modified active ribonuclease, RNase (S)l and its fractionation into two components, a peptide (S-peptide) and a protein (S-protein). Neither S-peptide nor S...