Canine epididymides were excised and immediately stored at 4 degrees C for 48 hr, and the qualities of caudal epididymal sperm after recovery and cryopreservation were evaluated. To confirm the fertility of the cryopreserved caudal epididymal sperm, artificial intrauterine insemination was performed. The sperm motility (61.0%) immediately after recovery from caudal epididymis stored at 4 degree...

Changes of chromosomal basic proteins of rats have been followed during transformation of spermatids into spermatozoa in the testis and during maturation of spermatozoa in the epididymis. Rat testis chromatin has been fractionated on the basis of differing sensitivity to shearing, yielding a soluble fraction and a condensed fraction. The sperm histone is found in the condensed fraction. Somatic...

Vasectomy reversal involves either vasovasostomy (VV) or epididymovasostomy (EV), and rates of epididymal obstruction and EV increase with time after vasectomy. However, as older vasectomies may not require EV for successful reversal, we hypothesized that sperm production falls after vasectomy and can protect the system from epididymal blowout. Our objective was to define how the need for EV at...

Binder of SPerm (BSP) proteins are a family of proteins expressed exclusively in the male reproductive tract (seminal vesicles or epididymis) of several mammalian species. They are known to promote capacitation, a sperm maturation step essential for fertilization. Our recent studies have shown that in human, the Binder of SPerm Homolog 1 (BSPH1) is expressed solely in epididymal tissues. The go...

Acquisition of fertilization ability by spermatozoa during epididymal transit occurs in part by the transfer of molecules from membranous vesicles called epididymosomes. Epididymosomes are heterogeneous in terms of both size and molecular composition. Exosomes and other related small membranous vesicles (30-120 nm) containing tetraspanin proteins on their surface are found in many biological fl...

BACKGROUND & OBJECTIVES Polychlorinated biphenyls (PCB), the synthetic chlorinated organic compounds, are known to decrease thyroid function, sperm count and fertility, and increase the risk of testicular cancer may cause serious effect on male reproduction. The objective of the present study was to study the effect of PCB, Aroclor 1254 on rat epididymal structure and function. METHODS Adult ...

Cisplatin (CIS) is used in the treatment of cancer, but its nonspecific systemic actions lead to toxic effects on other parts of the body. This study investigated the severity of CIS toxicity by increasing its dose over a constant time period. Sprague Dawley rats were divided into five treatment groups and control group with CIS (2, 4, 6, 8, and 10 mg/kg) administered intraperitoneally for 5 da...

Background: Alteration in the protein signatures of functionally immature testicular spermatozoa occurs during their journey through the epididymis. This leads to acquisition of sperm domain specific functions essential for successful fertilization. Epididymal sperm proteins are preferred targets for immunocontraception as well as in elucidating the causes of infertility. The Background of the ...

Quassia amara is a medicinal plant with several pharmacological properties. There are preliminary reports implicating this plant in male reproductive toxicology. Single daily oral administration of methanol extract of Quassia amara stem bark for 6 weeks resulted in a significant reduction in the weight of the testes, caudal epididymis and seminal vescicle. Caudal epididymal sperm parameters sho...

cell in biological studies. The studies showed the good response of human sperm to cryobiologic assays. However, in farm animals because of different composition of the cell membrane and presence of more unsaturated fatty acid make sperm more sensitive to oxidative stress that is one the most important factors that can affect the sperm viability during cryopreservation. The aim of the present s...