The electrophoretic banding patternof esterases were examined in six different tissues of Pomacea paludosa and Pomacea canaliculata by using different inhibitors viz: pCMB, Paraoxon, Physostigmine, EDTA and AgNo3. Same esterolytic active zones were observed in Ctenidia and Mantle of both species, the maximum number of bands exhibiting in Ctenidia of P.paludosa and P.canaliculata. Species specif...

FRET-based fluorogenic substrates for lipases and esterases were prepared in four steps from commercially available building blocks. The substrates are pyrenebutyric acid monoesters of aliphatic 1,2-diols bearing a dinitrophenylamino group as a quencher. The most enzyme-reactive substrate is ester 2a. The substrates do not show any measurable background reaction in the absence of enzyme even at...

p-Nitrophenyl acetate is the most commonly used substrate for detecting the catalytic activity of esterases, including those that activate prodrugs in human cells. This substrate is unstable in aqueous solution, limiting its utility. Here, a stable chromogenic substrate for esterases is produced by the structural isolation of an acetyl ester and p-nitroaniline group using a trimethyl lock moiet...