نتایج جستجو برای: eukaryotic expression system
تعداد نتایج: 3009342 فیلتر نتایج به سال:
background and objectives: some of the human papillomaviruses (hpvs) can infect genital tracts and are sometimes associated with anogenital tract cancers. hpvs induced cervical cancers through the expression of e6 and e7 genes by inactivating the tumor suppressor proteins. in this study, e6 and e7 genes were chosen in order to construct an expression vector which is able to express target prote...
Protein arginine methylation is a widespread post-translational modification (PTM) in eukaryotic cells. This chemical proteins functionally modulates diverse cellular processes from signal transduction, gene expression, DNA damage repair,...
Background: Apoptin is a protein from chicken anemia virus that could induce apoptosis specifically in the cancer cells but it has not any effect in the normal cells. Phage therapy is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. The bacter...
conclusions the results of the present study will increase our knowledge about molecular cloning and expression of the l. infantum kmp-11 gene, and this may be used as an effective target for controlling visceral leishmaniasis. results the kmp-11 gene was successfully subcloned in pcdna3 as a eukaryotic expression vector. recombinant kmp-11 protein was confirmed by the reverse transcriptase pol...
Background and Objectives: Some of the human papillomaviruses (HPVs) can infect genital tracts and are sometimes associated with anogenital tract cancers. HPVs induced cervical cancers through the expression of E6 and E7 genes by inactivating the tumor suppressor proteins. In this study, E6 and E7 genes were chosen in order to construct an expression vector which is able to express ...
Background and Objective: The glycoprotein hormone secreted from anterior pituitary gland are heterodimeric, non- covalently consisting of a common α subunit and a hormone-specific β subunit. Due to the lack of adequate access to hormones and the possibility of contamination, this study attempted to clone two subunits of LH hormone by using IRES sequence in eukaryotic expression vector pEGFP-NI...
objectives: in present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. materials and methods: we have cloned bovine prochymosin gene in methylotrophic yeast, p. pastoris, using ppic9k as an expression vector. the recombinant plasmid was transformed into the host by electroporation, and it was expressed in optimum conditions...
The paper summarizes the current status of the reporter gene technology and their basics. Reporter gene technology is widely used to monitor cellular events associated with gene expression and signal transduction. Based upon the splicing of transcriptional control elements to a variety of reporter genes, it “reports” the effects of a cascade of signaling events on gene expression inside cells. ...
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