The aim of this work was to develop a new method for constructing vectors, named ligation-independent cloning (LIC) method. We constructed the S label expression vector and recombinant pET32a (+) S-phoN2 by LIC. The recombinant proteins were expressed in E. coli at a high level, and then the specificity of the recombinant proteins was identified by western blot. The target band was detected by ...

this paper examines the nature of language in the works of mawlana jalal al-din rumi and consists of three sections: language and reality in rumi; the complex view of language in fihi ma fihi (the discourses of rumi); and, poetry and mysticism in rumi. the paper discusses three main topics: rumi's ambivalent attitude to language, which is seen as both a means of conveying truth and guidanc...

brassica napus is an important oilseed crop and the yield loss due to fungal disease stem rot caused by sclerotinia sclerotiorum is a serious problem in cultivation of this crop. the pathogenesis-related (pr) protein, glucanase, hydrolyzes a major cell wall component, glucan, of the pathogenic fungi and acts as a plant defense barrier. in this study, a β-1,3-glucanase (bgn13.1) gene was isolate...

  Objective(s): Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analy...