The objective of the present study was to determine the effect of non-enzymatic antioxidants (vitamin C or E) in tris-citric acid buffer (TCA) on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Split pooled buffalo bull ejaculates were diluted in TCA egg yolk glycerol extender containing either vitamin C (TCAC), vitamin E (TCAE) or without antioxidant (TCAN) at 37°C. Extended s...

The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic H...

This experiment wascarried out to explore the effect of use of combinations of permeating cryoprotectant and non permeating cryoprotectant on thequality of frozen-thawed Beluga (Huso huso) spermas compared to the use of permeating cryoprotectant as a control. For this work methanol (MeOH) and dimethyl sulfoxide (DMSO)are used aspermeatingcryoprotectantsand bovine serum albuminas a non permeatin...

The objective of this study was to evaluate the efficacy of EK extender alone or incorporation with CoenzymeQ10 (CoQ10) and/or soyphosphatidylcholine (SPC) in poultry semen and their effects on seminal traits during temporal storage at 40C for different time intervals (12 h, 24 h, and 36 h). Heterospermic pooled semen samples diluted (1:4) with EK, EK + SPC, EK+ CoQ10 and EK + SPC + CoQ10 exten...

On-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlation between sperm concentrations measured by the Nucleocounter SP-100 and by the Bürker counting chambe...

Slow-cooled stallion spermatozoa, with and without seminal plasma removed by centrifugation, were diluted in Kenney's extender (KE) containing nonfat dry skim milk with glucose and antibiotics or in KE supplemented by adding a modified high-potassium Tyrode's medium (KMT). Four ejaculates from each of four stallions were collected and divided factorially across these four treatments. Percentage...

The Punjab Urial (Ovis vignei punjabiensis) is an endangered subspecie of ovidae, distributed as small scattered populations in the forest belt of the Himalayan foothills of Pakistan and in the areas enclosed by the Indus and the Jhelum rivers. The present study was conducted to evaluate the liquid storage of Punjab Urial spermatozoa in different extenders for use in future in situ conservation...

The objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. Thirty ejaculates collected from ten Holstein bulls were pooled and evaluated at 37 °C. Pool ejaculated was split into two main experimental groups, 1 and 2. In experiment 1, specimen was diluted...

One of the basic concepts of set theoretic geology is the mantle of a model of set theory V: it is the intersection of all grounds of V, that is, of all inner models M of V such that V is a set-forcing extension of M . The main theme of the present paper is to identify situations in which the mantle turns out to be a fine structural extender model. The first main result is that this is the case...

Cryopreservation of semen from the Caspian brown trout (Salmo trutta caspius) and effects of extender type, freezing and thawing rates on fertilization ability were studied. After assessment of semen quality, one part of semen was diluted with three parts of different extender and decanted into 0.5ml straws. Freezing was carried out at two freezing levels, 1.5cm and 2cm above surface of liquid ...