Cellular systems are essential model systems to study reactive oxygen species and oxidative damage but there are widely accepted technical difficulties with available methods for quantifying endogenous oxidative damage in these systems. Here we present a stable isotope dilution UPLC-MS/MS protocol for measuring F2-isoprostanes as accurate markers for endogenous oxidative damage in cellular syst...

Fruit and vegetable consumption has been associated with a reduced risk of several diseases including CVD. A part of these effects seen could be linked to anti-inflammatory and antioxidative effects, although this has not been thoroughly investigated. The present study was designed to investigate the effects of the dietary intake of beta-carotene, alpha-tocopherol and ascorbic acid on in vivo b...

BACKGROUND Cigarette smoke is a major source of free radicals and oxidative stress. With a significant proportion of women still smoking during pregnancy, this common and avoidable exposure has the potential to influence infant oxidative status, which is implicated in the increased propensity for airway inflammation and asthma. The aim of this study was to examine the effects of maternal smokin...

Obesity affects over 40% of the adult population and is a major risk factor for morbidity mortality due to cardiovascular disease. Black women have one highest prevalences obesity, insulin resistance, hypertension, events in US. We previously found that free radical-mediated lipid peroxidation contributes IL-6 production dendritic cells leading inflammation hypertension. Thus, we hypothesized F...

See article by Wilson et al. [9] (pages 601 –607) in this attenuated by pretreatment with L-arginine or the NO issue. donor NOR-3. These observations suggest a tonic vasoconstrictor activity dynamically opposed by NO, with the net vasomotor effect resulting from the balance of the two

Context. Low levels of systemic F2-isoprostanes (F2-IsoP) increase the risk of diabetes and weight gain and were found in African Americans. Low F2-IsoPs could reflect an unfavorable metabolic characteristic, namely, slow mitochondrial metabolism in individuals with African ancestry. Objective. To examine differences in plasma F2-IsoPs in three groups with a priori different proportion of Afric...

The foregoing article was focused on the modulation by dietary flavonoids of generation and biological role of plasma lipoproteins and the consequences as to the risk for cardiovascular diseases. The objective of the second article of this series is to gain deeper insight into the cellular and molecular action of flavonoids and other dietary polyphenols and their bioactive species, respectively...