We report that the Gm7068 (CatSpere) and Tex40 (CatSperz) genes encode novel subunits of a 9-subunit CatSper ion channel complex. Targeted disruption of CatSperz reduces CatSper current and sperm rheotactic efficiency in mice, resulting in severe male subfertility. Normally distributed in linear quadrilateral nanodomains along the flagellum, the complex lacking CatSperζ is disrupted at ~0.8 μm ...

Background For patients with severe endometriosis,the spontaneous pregnancy rates have been reported to be near 0 due to extreme distortion of normal pelvic anatomy.Surgery is one of the treatment options;however.f,patients failed to conceive after surgerY,in vitro fertilization(IVF)Is effective.The objective of this retrospective study was to determine the clinicaI characteristics of IVF/intra...

The aim of this report has been evaluating protamine content and DNA integrity of two patients with globozoospermia undergoing Intra Cytoplasmic Sperm Injection (ICSI). Semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA fragmentation was assessed using Chromomycin A3 and sperm chromatin dispersion assay respectively. ICSI and chemical activation were carried ...

introduction: the frequency of sperm pcc indiction in normal and infertile men was investigated. material and methods: zona – free hamster oocyte were retrieved after super ovulation by pmsg and hcg infection. following treatment with hyaloronidase, zona was removed by trypsin digestion. sperms were classified with sperms, and then transferred to fresh media containing colcemid. slides were pre...

Numerical chromosome aberrations in gametes typically lead to failed fertilization, spontaneous abortion or a chromosomally abnormal fetus. By means of preimplantation genetic diagnosis (PGD), we now can screen human embryos in vitro for aneuploidy before transferring the embryos to the uterus. PGD allows us to select unaffected embryos for transfer and increases the implantation rate in in vit...

The aim of this study was to compare the sperm protein expression profile (proteome map) from a patient who experienced failed fertilization at IVF with fertile controls. One patient and three fertile donor sperm samples were characterized using two-dimensional electrophoresis. Differences in protein expression were established using gel analysis software before attempted protein identification...

Many infertility clinics program ICSI directly in their IVF treatment plans due to the fear of failed or poor fertilization rates. A number still however perform conventional insemination. To aid patients who fail insemination, “rescue ICSI” is sometimes performed on oocytes that fertilize, allowing salvage cycle. This study analyzes pregnancy outcomes were able receive rescue ICSI; more specif...

Variation in fertilization success underlies sexual selection, yet mating does not guarantee fertilization. The relationship between natural inseminations and fertilization success is essential for understanding sexual selection, yet that relationship and its underlying mechanisms are poorly understood in sperm-storing vertebrates such as birds. Here the relationship is analyzed in mating trial...

Previous cases reported recurrent all oocytes in repeated cycles were arrested at MI failed to mature after culture and ICSI resulted total nonfertilization. Ionomycin induced first PB extrusion patients with maturation-resistant oocytes. combined assisted oocyte activation (AOA) on MII matured vitro abnormal fertilization no further embryo cleavage potential. The objective of this work was res...

OBJECTIVE Determine whether testicular sperm extractions and pregnancy outcomes are influenced by male and female infertility diagnoses, location of surgical center and time to cryopreservation. PATIENTS One hundred and thirty men undergoing testicular sperm extraction and 76 couples undergoing 123 in vitro fertilization cycles with testicular sperm. OUTCOME MEASURES Successful sperm recove...