11-23 ABSTRACT A complementary DNA (cDNA) library was constructed from the mRNA of adult worms of Fasciola hepatica in the expression vector λgt11, the size of the library contained approximately 2.4 x 10 recombinants and the recombinant efficiency was 85%. The library was directedly screened with a rabbit antisera raised against a excretory-secretory (ES) antigen of Fasciola hepatica. Two posi...

background : in this study, we used both its1 and its2 for molecular identification of fasciola species. methods : the region between 18s and 28s of ribosomal dna was used in pcr-rflp method for molecular identification of fasciola species. ninety trematodes of fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from khorasan, east azerbaijan, a...

We report the first evidence of natural infection of Lymnaea columella with Fasciola hepatica in Argentina. A sample of 601 snails was collected in May 2003 in northeastern Corrientes, a province bounded on the north by Paraguay, on the east by Brazil and on the southeast by Uruguay. Among 500 examined snails, 44 (8.8%) were exclusively infected with F. hepatica. Parasite identification was bas...

BACKGROUND Fascioliasis, caused by Fasciola hepatica and F. gigantica, is one of the most important plant and water borne disease in Iran. This study aimed to determine the prevalence of fascioliasis and intestinal helminthes in inhabitants of rural areas of Boyer-Ahmad in Kohgiluyeh and Boyer-Ahmad province in Southwest of Iran. METHODS Stool samples (1025) were collected from inhabitant of ...

background: fascioliasis caused by fasciola hepatica and f. gigantica is a foodborne and waterborne zoonotic disease with a worldwide distribution. the illness occurs in regions with intensive sheep or cattle production. in some parts of iran the incidence of human infection has increased over the past decades. since chaharmahal and bakhtiyari province has been known as a main pole of tradition...

background: the purpose of this comparative study was to detect superoxide dismutase (sod) activities in fasciola hepatica, f. gigantica parasites, infected and healthy liver tissues in order to determine of species effects and liver infection on sods activity level. methods: fasciola spp. parasites and sheep liver tissues (healthy and infected liver tissues), 10 samples for each, were collecte...

Hepatic fascioliasis is a parasitic disease caused by the fluke Fasciola hepatica. In chronic phase of disease, adult form parasite adopts bile ducts as ideal environment to develop, causing them dilate and simulating an obstructive jaundice process. The case 48-year-old woman presented, who was admitted for jaundice, choluria eight loss. After corresponding complementary studies had been carri...

The current study aimed to find out the morphometric and genotypic divergences of the flukes isolated from different hosts in a newly emerging focus of human fascioliasis in Iran. Adult Fasciola spp. were collected from 34 cattle, 13 sheep, and 11 goats from Kohgiluyeh and Boyer-Ahmad province, southwest of Iran. Genomic DNA was extracted from the flukes and PCR-RFLP was used to characterize th...

During an excavation of a site of the corded ware culture in the Saale-Unstrut-Valley (ca. 3000 BC) in Germany, a soil sample from the pelvis of a human skeleton was studied under palaeoparasitological aspects. Eggs of the trematode Fasciola hepatica and of the nematode genus Capillaria were found. This is the first case of a direct association of a F. hepatica-infestation to both a prehistoric...