Meningitis is a severe and potentially fatal form of tuberculosis. The diagnostic workup involves detection of acid-fast bacilli (AFB) in the cerebrospinal fluid (CSF) by microscopy or culture, however, the difficulty in detecting the organism poses a challenge to diagnosis. The use of the polymerase chain reaction (PCR) in the diagnostic approach to Mycobacterium tuberculosis (MTB) meningitis ...

BACKGROUND The polymerase chain reaction (PCR) assay, introduced as a fast and sensitive diagnostic method, has been known to be useful in detecting Mycobacterium tuberculosis. Therefore the purpose of this study was to evaluate the usefulness of an in-house PCR assay in the detection of Mycobacterium tuberculosis by comparing PCR results with those of conventional diagnostic techniques. METH...

The GABA projection neurons in the substantial nigra pars reticulata (SNr) are key output neurons of the basal ganglia motor control circuit. These neurons fire sustained high-frequency, short-duration spikes that provide a tonic inhibition to their targets and are critical to movement control. We hypothesized that a robust voltage-activated K(+) conductance that activates quickly and resists i...

american foulbrood (afb) is the most serious brood disease of the honey bee. traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. the aim of this study is the detection of paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and pcr. therefore 54 samp...

Abstract As the COVID-19 infection continues to ravage world, advent of an efficient as well economization existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help management pandemic. This study demonstrated innovative rapid corroboration test on multiplex PCR. An assessment optimal PCR conditions simultaneously amplify SARS-CoV-2 ge...

Advent and fast spread of pandemic diseases draw worldwide attention to rapid, prompt, accurate molecular diagnostics with technical development ultrafast polymerase chain reaction (PCR). Microfluidic on-chip PCR platforms provide highly efficient small-volume bioassay for point-of-care diagnostic applications. Here we report ultrafast, real-time, nanoplasmonic rapid quantitative at level. The ...

Many allergens, such as hazelnut, peanut, charlock, celery, sesame, lupine, walnut, almond, macadamia nut, hickory, pistachio, wheat gliadins, may be present in food products, however, undeclared or as unintentional additives. Due to the growing number of allergic reactions, it is crucial to have fast, reliable methods of allergen detection in processed food products. This review summarizes the...

the dna molecule has been known to be the cellular target for many cytotoxic anticancer agents for several decades. understanding how drug molecules interact with dna has become an active research area in the interface between chemistry, molecular biology and medicine. dna extraction has been suggested as a main step affecting molecular dna technology such as pcr and pcr-based methods. therefor...

The basis for the identification of SARS-CoV-2 cases in handling COVID-19 pandemic is Reverse Transcriptasi Polymerase (RT-PCR) and specific rapid antigen test. RT-PCR test takes a long time expensive to get results, while simple fast alternative getting detection results. method used descriptive observational form literature review by reviewing existing literature. Several screening confirmato...