Perennial ryegrass (Lolium perenne L.) and tall fescue (Festuca arundinacea Schreb.) are two major turfgrass species in the United States. Their improvement via biotechnology approaches depend on the tissue culture response, particularly in vitro plant regeneration. To understand more of the regeneration event, scanning electron microscopy was performed and somatic embryogenesis was revealed in...

Biotechnology approaches can supplement conventional breeding efforts for turfgrass improvement. The objective of this study was to develop reliable transformation and selection schemes for elite turf-type tall fescue cultivars. Transgenic tall fescue (Festuca arundinacea Schreb.) plants were obtained by microprojectile bombardment of embryogenic suspension cells of two elite turf-type cultivar...

Tall fescue (Festuca arundinacea Schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. To develop transgenic tall fescue plants with enhanced tolerance to abiotic stress, we introduced an alfalfa Hsp23 gene expression vector construct through Agrobacterium-mediated transformation. Integration and expression of the transgene were confi...

Heat stress is a critical challenge to tall fescue (Festuca arundinacea Schreb.) in many areas of the globe and variations in genetic structure and functional traits is for the efficient breeding programs on developing heat tolerant cultivars. Tolerant-related morpho-physiological traits and simple sequence repeat (SSR) markers were employed to survey genetic diversity in greenhouse and growth ...

A growing-finishing study using Angus steer calves was conducted in three phases: 1) grazing stockpiled 'Kentucky-31' tall fescue (Festuca arundinacea Schreb.) with high (65%; HE KY-31) and low (0%; LE KY-31) infestation rates of Acremonium coenophialum Morgan-Jones and Gams and 'Kenhy' and 'Johnstone' tall fescue with low (< 1%) infestation rate of Acremonium coenophialum from October 24 to De...

Tall fescue (Festuca arundinacea Schreb.) is widely utilized as a major forage and turfgrass species in the temperate regions of the world and is a valuable plant material for studying molecular mechanisms of grass stress tolerance due to its superior drought and heat tolerance among cool-season species. Selection of suitable reference genes for quantification of target gene expression is impor...