Nucleic acids in young leaves of Swiss chard have been studied by light and electron microscope techniques. Leaf DNA has also been characterized by density gradient centrifugation and shown to contain a minor band of higher guanine plus cytosine (GC) content, presumably attributable to chloroplasts. The chloroplasts were faintly stained by the Feulgen reaction; radioautography demonstrated the ...

SUMMARY The incidence of renal carcinoma in frogs maintained in the laboratory was not significantly affected by the inoculation of cell suspensions or cell-free filtrates of tumors. There was no significant difference in the tumor incidence in experimental or control frogs whether maintained individually or in groups of ten. Histological sections of 61 tumors stained with Feulgen and fast gree...

Micronuclei in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. To elucidate the effect of different staining procedures on the outcome of such investigation, we conducted a study in which the micronuclei frequencies in oral mucosa cells of heavy smokers (n = 20) and nonsmokers (n = 10) were evaluated with nonspecific (Giemsa, May-Grünwald-Giemsa) and DNA-spec...

The aim of the study was to identify pathomorphology brain damage in patients who died COVID-19. Material and methods . Autopsy reports autopsy material 17 deceased with pre-mortem confirmed COVID-19 infection were analyzed. Fatal cases which COVID19 major cause death included study. Five people diagnosed cerebral infarction. Organ samples taken for histological examination during autopsy. Sect...

The chemical test for deoxyribosenucleic acid discovered by Feulgen (1914) and applied by Feulgen & Rossenbeck (1924) under the name of nucleal stain to histological sections has served many useful purposes in the hands both of its originator and of subsequent workers. It now finds much favour as a staining process for cytological preparations containing dividing cells, partly, no doubt, becaus...

To simultaneously visualize individual cell nuclei and tissue morphologies of the zebrafish retina under bright field light microscopy, it is necessary to establish a procedure that specifically and sensitively stains the cell nuclei in thin tissue sections. This necessity arises from the high nuclear density of the retina and the highly decondensed chromatin of the cone photoreceptors, which s...

Dyskinetoplastic cells from both Crithidia fasciculata and Trypanosoma equiperdum lack detectable kinetoplast DNA (kDNA) by conventional staining techniques. Two dyskinetoplastic strains of T. equiperdum, either acriflavine-induced or spontaneously occurring, show normal amounts of kDNA (p = 1.692 g/cm3) in analytical caesium chloride, ultracentrifugation. Electron and fluorescence microscopy o...