Recently, there has been a great interest in melanoma and stromal cell-to-cell interactions behind the progressive behavior of melanoma. By vitro co-culturing using sequential seeding conditions imitating niche properties, gained hybrid variants are visualized by high content fluorescent microscopy. The co-culture experiment applied consecutive order primarily plated HDF-RFP-ZeoR fibroblast mon...

A central issue in tumor biology is the understanding of the interactions between tumor cells and their environment. Using normal and ras oncogene transfected rat fibroblast cells, we now demonstrate that the transfected cells make altered extracellular matrices (ECM) and that their resulting ECM influence the proliferation and genetic regulation of human bladder cancer EJ cells. Using Western ...

ethanol known as ethyl alcohol is being widely used around the world. many serious diseases are related with its consumption.  alcohol posses many divers effects on human body including risk of cirrhosis and/or hepatocellular carcinoma. therefore, analysis of this component is prominent. fibroblast cells were cultured in various dosages of ethanol. the effective dosage was then investigated by ...

Tumour growth is angiogenesis-dependent; brain tumours have more intense neovascularisation than other tumours and produce basic fibroblast growth factor, a potent angiogenic mediator. Because little is known about the release of basic fibroblast growth factor from brain tumours into extracellular fluids, we tested cerebrospinal fluid (CSF) from 26 children and young adults with brain tumours a...

background: the multifunctional transforming growth factor beta (tgf-β) is a glycoprotein that exists in three isoforms. tgf-β3 expression increases in fetal wound healing and reduces fibronectin and collagen i and iii deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. fibroblasts are key cells in t...