Catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography (MICRO-CARD-FISH) is increasingly being used to obtain qualitative information on substrate uptake by individual members of specific prokaryotic communities. Here we evaluated the potential for using this approach quantitatively by relating the measured silver grain area around cells taking up (3...

The objective of this paper focused on the ffects of dietary nucleotides (NT) on histopathological alternations of striped catfish Pangasianodon hypophthalmus, after exposure to water-borne silver nanoparticles (AgNPs) and silver nitrate (AgNO3). Fish were fed with a diet containing nucleotide (0.75%) or control diet for 10 weeks and then divided into 3 experiments including control, 20 µ...

The importance of rainbow trout (Onchorhynchus mykiss) in Iran aquaculture industry on one hand, and increasing the mortality of this fish due to outbreaks of infectious diseases, on the other hand, indicate the requirement for more profound understanding the rainbow trout immune system and access to laboratory tools for definitive diagnosis of its diseases. One of the most important defense me...

The aim of this project was to compare cerium oxide and silver particles of different sizes for their potential for uptake by aquatic species, human exposure via ingestion of contaminated food sources and to assess their resultant toxicity. The results demonstrate the potential for uptake of nano and larger particles by fish via the gastrointestinal tract, and by human intestinal epithelial cel...

Collagen, a major component of native extracellular matrix, has diverse biomedical applications. However, its application is limited due to lack of cost-effective production and risk of disease transmission from bovine sources currently utilized. This study describes fabrication and characterization of nano/micro fibrous scaffolds utilizing collagen extracted from fresh water fish origin. This ...

Adult brown trout (300­600 g) were acclimated for 2 weeks to an artificial soft water (Ca2+, 50 µmol l-1) and maintained at either 5 °C (October to March) or 15 °C (May to August). Following insertion of a cannula into the dorsal aorta under MS-222 anaesthesia and a recovery period of 2 days, the fish were exposed to a 4 day episode of sub-lethal copper levels at pH 5 or kept ...

Aging Antarctic icefish is difficult because of their lack scales and poorly calcified bones. Icefish ages must therefore be estimated from otoliths. We describe a method reading daily micro-increments in connection with shape, size mass analyses the otoliths South Georgia Pseudochaenichthys georgianus . Changes otolith morphology correlate fish age group. The micro-increment analysis capable e...

In the aftermath of the Paris Agreement, there is a crucial need for scientists in both thermal physiology and climate change research to develop the integrated approaches necessary to evaluate the health, economic, technological, social, and cultural impacts of 1.5°C warming. Our aim was to explore the fidelity of remote temperature measurements for quantitatively identifying the continuous re...