Lasers are the principle light sources of flow cytometers. Most modern benchtop instruments feature air-cooled argon-ion laser emitting 10-25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers. Also included is a discussion of future directions, particularly in the development of solid-...

Cell Analysis Recent advances in molecular and cell biology—the study of cells including their function, the structures they contain, their interactions with their environment, and their life cycle, division, and death—have helped researchers gain a deeper understanding of human disease. Advancing understanding of the different types of cells populations, how cells work, and the differences bet...

purification and isolation of cellular target proteins for monoclonal antibody (mab) production is a difficult and time-consuming process. immunization of mice with murine cell lines stably transfected with genes coding for xenogenic target molecules is an alternative method for mouse immunization and mab production. here we present data on transfection efficiency of some commercial reagents us...

Background: Chronic myelogenous leukemia (CML) is a myeloproliferative disorder due to BCR-ABL1 translocation. Patients showing transformation into blast crisis (BC) have poorer treatment response and prognosis.  Methods: A retrospective study was conducted in the department of Pathology, Maulana Azad Medical College, New Delhi, India, over a time period of 5 years (2014-2019) to evaluate the ...

• Introduction: Microbial Flow Cytometry: Technology and Applications. Martin G. Wilkinson • Chapter 1: Microbial Cytometry: What It Was, Is, and May Be. Howard M. Shapiro • Chapter 2: Non-Destructive OnChip Imaging Cytometry Assay for Constructive On-Chip Cellomics Studies. Kenji Yasuda • Chapter 3: Application of Flow Cytometry to Environmental Biotechnology. Mónica Herrero and Mario Díaz • C...

BACKGROUND Elasticity of cells is determined by their cytoskeleton. Changes in cellular function are reflected in the amount of cytoskeletal proteins and their associated networks. Drastic examples are diseases such as cancer, in which the altered cytoskeleton is even diagnostic. This connection between cellular function and cytoskeletal mechanical properties suggests using the deformability of...

Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...