The phenolic pKa of fluorescein varies depending on its environment. The fluorescence of the dye varies likewise. Accordingly, a change in fluorescence can report on the association of a fluorescein conjugate to another molecule. Here, we demonstrate how to optimize this process with chemical synthesis. The fluorescence of fluorescein-labeled model protein, bovine pancreatic ribonuclease (RNase...

A novel fluorescein-bridged perylene bisimide (PBI) dimer for liquid crystal (LC) with geometrically symmetric structure was developed. The mesomorphic results indicated that the energetically stable and unstable conformers of fluorescein fragments could lead to transformation mesophases from a hexagonal columnar mesophase an uncertain phase at 136.9 °C in heating, whilst maintained between 175...

BACKGROUND AND OBJECTIVES The growth and progression of ovarian tumor metastases at the peritoneal surfaces of the abdominal cavity are coupled with neovascularization. Newly formed tumor vessels show a more diffuse pattern and are more permeable for macromolecules than normal vessels. We investigated the possibility to detect (small) ovarian metastases in the abdominal cavity by means of fluor...

SIRs,-In your April issue (Brit. J. Ophthal., 1965, 49, 205) you published my paper "Applanation Tonometry using One Drop of an Anaesthetic-Fluorescein Mixture". This paper recommended a mixture containing 0 3 per cent. Novesine and 0)125 per cent. fluorescein; one drop gave good corneal anaesthesia and a good fluorescent ring for Goldmann applanation tonometry. Of the eight anaesthetic solutio...

We report a new method for quantitating retinal blood vessel width from fluorescein angiogram negatives using a computerized image analyser. Interuser and intrauser variability were 3 to 4 times lower than reported with older methods. In addition, model blood vessels were used to test the accuracy of our method under four clinically relevant conditions: variations in vessel width, fluorescein c...

Multiphoton microscopy has been shown to be a useful tool in studying drug distribution in biological tissues. In addition, fluorescence lifetime imaging provides information about the structure and dynamics of fluorophores based on their fluorescence lifetimes. Fluorescein, a commonly used fluorescent probe, is metabolized within liver cells to fluorescein mono-glucuronide, which is also fluor...

The mechanism of intracellular fluorescein accumulation was investigated by studying fluorescein accumulation kinetics in individual cells in culture. The experiments were done with K562 human erythroleukemia cell culture and a primary culture of human embryonic skin fibroblasts. Various experimental conditions were used by varying pH and ion composition of the external media, and/or temperatur...