Novel copper(II) coordination compounds with chiral macrocyclic imine ligands derived from R-/S-camphor were asymmetrically synthesized and characterized with the aid of chiroptical spectroscopies. Crystal structures of both enantiomers were determined by single crystal X-ray diffraction analysis. Circular dichroism (CD) spectra were analyzed using a simplified exciton model as well as quantum ...

The interaction between [Pt(phen)(pyrr-dtc)]NO3 (where phen = 1,10-phenanthroline and pyrr-dtc = pyrrolidinedithiocarbamat) with human serum albumin (HSA) was studied by fluorescence, UV-Vis absorption, circular dichroism (CD) spectroscopy and molecular docking technique under like physiological condition in Tris-HCl buffer solution at pH 7.4. UV-Vis absorption spectroscopy indicates that the p...

Fanconi anemia complementation groups - I (FANCI) protein facilitates DNA ICL (Inter-Cross-link) repair and plays a crucial role in genomic integrity. FANCI is a 1328 amino acids protein which contains armadillo (ARM) repeats and EDGE motif at the C-terminus. ARM repeats are functionally diverse and evolutionarily conserved domain that plays a pivotal role in protein-protein and protein-DNA int...

The potential of fluorescence-detected circular dichroism (FDCD) to extend the scope of the exciton chirality method is rooted in the increased sensitivity and selectivity of fluorescence as compared with absorbance measurements. In this paper, several practical aspects of FDCD are addressed with model compounds possessing a (1R,2R)-trans-1,2-cyclohexanediol skeleton and two chromophores attach...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...

Binding of gefitinib (GEF), a promising anticancer drug to human serum albumin (HSA), the major transport protein in blood circulation was investigated using fluorescence, UV-vis absorption and circular dichroism (CD) spectroscopy as well as computational modeling. Fluorescence quenching of HSA upon GEF addition was found to be a static quenching process, as revealed from the decreasing trend o...

Human serum albumin (HSA) is an abundant, multifunctional and nonglycosylated negatively charged plasma protein. HSA ascribed ligand-binding and transport properties, antioxidant functions and enzymatic activities. In the present study, the interaction and side effects of a new designed anti-cancer compound (1,10-phenanthroline butyl dithiocarbamato palladium(II) nitrate) on HSA have been inves...

We have studied the conformational stability of the two homologous membrane skeletal proteins, the erythroid and non-erythroid spectrins, in their dimeric and tetrameric forms respectively during unfolding in the presence of urea and guanidine hydrochloride (GuHCl). Fluorescence and circular dichroism (CD) spectroscopy have been used to study the changes of intrinsic tryptophan fluorescence, an...

Introduction Circular dichroism (CD) is the most widely used form of chiroptical spectroscopy, spectroscopic techniques that utilize the differential interaction of molecules with left− and right−circularly polarized light. In the absence of a magnetic field, the molecule must be chiral to give rise to a difference in the interaction with the two types of circularly polarized light. The phenome...