We present a new method of fluorescence imaging, which yields nm-scale axial height determination and ~15 nm axial resolution. The method uses the unique spectral signature of the fluorescent emission intensity well above a reflecting surface to determine vertical position unambiguously. We have demonstrated axial height determination with nm sensitivity by resolving the height difference of fl...

In order to study the effects of desiccation on a photosynthetic system, light harvesting and light-induced electron transport processes were examined in pea cotyledons at various moisture levels, using in vivo fluorescence excitation spectra and fluorescence induction kinetics. Water sorption isotherms yielded thermodynamic data that suggested very strong water binding between 4 to 11% water, ...

The paper discussed the preparation of rare earth europium complexes ultraviolet rays (UV) photochromic ink and the influences of factors, such as europium complexes using a rare earth ternary complex of Eu ion with 3-benzoylbenzoic acid and phenathrolinephen [Eu(BA)3Phen] content upon printing ink formula. In the experiment, we utilized fluorescence spectrophotometer to make quantitative deter...

Anharmonic effects of the absorption and fluorescence spectra of pyridine molecule are studied and analyzed for the two-low lying singlet excited states S(1)((1)B(1)) and S(2)((1)B(2)). The complete active space self-consistent field (CASSCF) method is utilized to compute equilibrium geometries and all 27 vibrational normal-mode frequencies for the ground state and the two excited states. The p...

With many aromatic compounds in liquid solution, the population of the metastable triplet state T, leads to delayed Sn-+SO fluorescence ( DFn, n 3 1) of the pyrene type, which results from the generation of excited singlet states S, by nonradiative triplettriplet annihilation (TTA) T, +T, +,+$. Knox and Swenberg have postulated that, apart from the DFl, there should be an extremely weak direct ...

A method for canopy Fluorescence Spectrum Reconstruction (FSR) is proposed in this study, which can be used to retrieve the solar-induced canopy fluorescence spectrum over the whole chlorophyll fluorescence emission region from 640–850 nm. Firstly, the radiance of the solar-induced chlorophyll fluorescence (Fs) at five absorption lines of the solar spectrum was retrieved by a Spectral Fitting M...

6MAP is a fluorescent analogue of adenine that undergoes Watson-Crick base pairing and base stacking in double-stranded DNA. The one-photon absorption spectrum of 6MAP is characterized by a maximum around 330 nm with moderate quantum yield fluorescence centered at about 420 nm. To take advantage of this probe for confocal and single-molecule microscopy, it would be advantageous to be able to ex...

Today, fluorescence imaging spans wide spectrum in modern biological investigations from molecular to network level at submicron resolution. To take advantage of full potential of advanced fluorescence imaging techniques, it is important to understand some underlying physical principles. This chapter provides a framework to understand basic principles underlying fluorescence, fluorescence micro...