We report on two ASCA observations of the high-mass X-ray binary pulsar OAO 1657−415. A short observation near mid-eclipse caught the source in a lowintensity state, with a weak continuum and iron emission dominated by the 6.4-keV fluorescent line. A later, longer observation found the source in a high-intensity state and covered the uneclipsed through mid-eclipse phases. In the high-intensity ...

in order to determine the cytotypes of 67 accessions of aegilops crassa from iran, two flow cytometry and cytogenetics methods were applied. the nucleus of the young leaves was colored using dapi and the intensity of fluorescent determined by flow cytometry. by comparing the intensity of the fluorescent of the ae. crassa samples with ae. tauschii (as an standard sample), two cytotypes 4x and 6x...

Standard fluorescence microscopy approaches rely on measurements at single excitation and emission bands to identify specific fluorophores and the setting of thresholds to quantify fluorophore intensity. This is often insufficient to reliably resolve and quantify fluorescent labels in tissues due to high autofluorescence. Here we describe the use of hyperspectral analysis techniques to resolve ...

We have developed a novel, non-intrusive fluid velocity measurement method based on photobleaching of a fluorescent dye for microfluidic devices. The residence time of the fluorescent dye in a laser beam depends on the flow velocity and approximately corresponds to the decaying time of the photobleaching of the dye in the laser beam. The residence time is inversely proportional to the flow velo...

A simple and Reliable Bench top microscope is constructed to detect the fluorescence intensity for quantum dot. Which can act as fluorescent tag a sensitive studies like sandwich enzyme-linked immunosorbent assay (ELISA). The Cadmium Telluride (CdTe) capped with MPA (3-Mercaptopropionic acid) dot signal output (Excitation: 490 nm, Emission: 515 nm) used. It bifunctional molecule containing both...

The sensitivity of chemical or biological analyses using fluorescent tagged reagents is limited by concentration quenching of the fluorescence at high tag concentrations and by photochemical bleaching if high illumination intensities are used. This paper shows by theoretical analysis and experimental verification that concentration quenching lengthens bleaching lifetimes, and the integrated flu...