PURPOSE To take advantage of specialized microscopic methods and transgenic stocks, to understand the properties of each rhodopsin now that Drosophila's six rhodopsins (Rh1-Rh6) have been isolated. METHODS The visual pigment containing organelles, the rhabdomeres, were imaged in live flies with the pseudopupil in standard and confocal microscopes. Five transgenic Drosophila strains in which R...

Lentivirus-based gene delivery vectors carrying multiple gene cassettes are powerful tools in gene transfer studies and gene therapy, allowing coexpression of multiple therapeutic factors and, if desired, fluorescent reporters. Current strategies to express transgenes and microRNA (miRNA) clusters from a single vector have certain limitations that affect transgene expression levels and/or vecto...

during 2007 – 2008 different fruit gardens in shiraz (fars province, iran) were surveyed for samples being collected from infected apple, pear and quince trees. symptoms of the infected trees included water soaking of infected tissue, wilt and tissue necrosis, scorched and blackend of blossom bracts or young shoots. a total of 47 strains were isolated from infected trees using na medium. on the...

Light-harvesting pigment-protein complexes of photosystem II of plants have a dual function: they efficiently use absorbed energy for photosynthesis at limiting sunlight intensity and dissipate the excess energy at saturating intensity for photoprotection. Recent single-molecule spectroscopy studies on the trimeric LHCII complex showed that environmental control of the intrinsic protein disorde...

rhizosphere soil samples were collected from olive orchards of golestan, guilan, zanjan and qom provinces. fluorescent pseudomonad strains were isolated from the collected samples on king’s b medium and their phenotypic features characterized as based on the standard bacteriological methods. for all the tested strains oxidase reaction, fluorescent pigment, growth at 4?c and arginine dihydrolase...

Fluorescent pigments in several Indo-Pacific and Caribbean anthozoans have recently been identified as proteins related to the green-fluorescent protein (GFP) of the hydromedusa Aequorea victoria. Here we show that GFP is widely distributed in many Caribbean species. The fluorescence excitation and emission spectra for the pigment are similar to those reported elsewhere for coral and noncoral G...

Fundus autofluorescence (FAF), a relatively new imaging modality, focuses on the fluorescent properties of pigments in the retina to generate images that help us view various disease processes from a different perspective. It aids us in the understanding of the pathophysiology of different retinal disorders. Recently, FAF imaging is being used commonly to help us in the diagnosis, prognosis as ...

This clinico-pathological study has been carried out on benign and malignant choroidal melanomata with the following objects in view: (I) To find out the fluorescent pattern on fluorescence angiography which would distinguish a pigmented choroidal malignant melanoma from other lesions of similar appearance in the fundus of the eye, e.g. pigmented benign choroidal melanoma or nonneoplastic pigme...

The effect of UV-C radiation on thylakoid arrangement, chlorophyll-a and carotenoid content and nitrogenase activity of the cyanobacterium Microchaete sp. was studied. Chlorophyll-a and carotenoid content increased gradually up to 48 h of UV-C exposure but declined with longer exposures. Nitrogenase activity decreased moderately with 6 to 12 h exposure and decreased substantially afterwards. Wh...

The microtubule system of melanophores of the angelfish, Pterophyllum scalare, has been studied using antibodies prepared against purified porcine brain tubulin in indirect immunofluorescence microscopy. Melanophores were freed from the surrounding tissue components of isolated scales by mild enzymatic digestion and then allowed to settle on a glass cover slip. In both the dispersed and the agg...