A high-performance liquid chromatographic (HPLC) method is described for the determination of duloxetine hydrochloride in capsules. The method was based on pre-column derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole using the fluorimetric detection technique. Duloxetine hydrochloride was analyzed by HPLC using an Inertsil C18 column (5 μm, 150 × 4.6 mm) and mobile phase consisted of ...

This paper reports on the development of an open-closed configuration for continuous kinetic methods based on photochemical reactions; the configuration removes the need to use special flow cells for simultaneous irradiation and detection. The sample plug was recirculated through a circuit including the detector and a coil irradiated with an ultra-violet source. A multipeak recording per inject...

1. A solvent system was devised for the extraction of isoniazid and its metabolites acetylisoniazid, monoacetylhydrazine, diacetylhydrazine, isonicotinic acid and isonicotinylglycine from serum and urine. 2. Specific chemical and fluorimetric methods were developed for the determination of the extracted isoniazid and acetylisoniazid, and chemical methods for the determination of monoacetylhydra...

stan-2b ,16b-ylene)bis(1-methyl-piperidinium) dibromide [15500-66-0] is general anaesthesia used in surgery for muscle relaxation and as an aid to endotracheal intubation (Chart 1). Several methods of determination of this drug have been reported in the literature, including high performance liquid chromatography (HPLC), capillary gas chromatography using nitrogen sensitive detection, liquid ch...

background: uranium poses both chemical and radiological hazard to the living system. drinking water from river is one of the major sources of uranium intake. dissolution of minerals, washout from rain water, rock-water interaction, agricultural run off, and industrial disposals are some of the sources of uranium in river water system. present study was aimed to determine the uranium in the wat...

A very simple, rapid and highly sensitive fluorimetric method for the determination of difhmisal in serum and urine is described. The method is based on the formation of a ternary complex between ditlunisal, Tb3+ and EDTA in alkaline aqueous solutions. This complex exhibits very intense terbium ion luminescence with a main emission maximum at 546 nm when excited at 284 mn. Optimum conditions fo...

The tripeptide Hip-His-Leu was used to standardize a fluorimetric method to measure tissue angiotensin-converting enzyme (ACE) activity in rats. The fluorescence of the o-phthaldialdehyde-His-Leu adduct was compared in the presence and absence of the homogenate (25 microl) to determine whether the homogenate from different tissues interfered with the fluorimetric determination of the His-Leu pr...

A sensitive and accurate method for the spectrofluorimetric determination of trace levels of aluminum in hemodialysis solutions using Mordant Red 19 as the complexation reagent has been developed. The optimal experimental conditions for the concentration of fluorimetric reagent, pH, temperature, and the specific type of matrix are reported. The emission of the fluorescent metal chelate was meas...

A simple, rapid and automatic fluorimetric method for the determination of total ascorbic acid is described. The method makes use of the stopped-flow mixing technique in order to achieve the rapid oxidation of ascorbic acid by dissolved oxygen to dehydroascorbic acid, which then reacts with o-phenylenediamine to form a fluorescent quinoxaline. The initial rate and fluorescence signal of this sy...