more than 80% of the total milk protein contains caseins that are to forms α-s1, α-s2, β-casein and kappa casein. kappa casein is smaller than other milk proteins but due to have a role in size and stability of micelles. the present study describes polymorphism of kappa casein gene. this is the first study of kappa casein gene polymorphism in najdi cattle of iran. we used the polymerase chain r...

a total of 125 russian red pied cows were genotyped for the prolactim-related gene. the prl-rsai genotypes were analysed using the polymerase chain reaction -restriction fragment length polymorphism (pcr-rflp) method. in this breed, the frequencies of alleles were as follows;  a= 0.794 and b= 0.206. the frequencies of aa, ab and bb genotypes were 0.598, 0.392 and 0.01; respectively. results sho...

Source/Description: We recently found a new polymorphism in the CYP1A1 gene by PCR direct sequencing (1) which results in amino acid substitution of De for Val at residue 462 in the heme binding region of the P450IA1 protein. This polymorphism asociates with Mspl polymorphism (2, 3) in the 3'-flanking region, and the linkage disequilibrium is calculated to be 0.158 (1). Here we report the PCR d...

The dataset presented in this article is related to the research article entitled "Detection of HER2 Gene Polymorphism in Breast Cancer: PCR Optimization Study" (B.R. Budiarto, Desriani, 2016) [1] with some modification in primers used and in PCR optimization strategy to eliminate false-positive result that may occur in HER2I655V polymorphism detection. Combining a new set of primers with PCR g...

Calreticulin (CALR) mutations are included in the WHO diagnostic criteria of Myeloproliferative Neoplasm (MPN). Presence CALR mutation, aids their diagnosis by excluding reactive causes. There several molecular techniques to detect mutations; however, these require advanced equipment and technical expertise. An in-house “polymerase chain reaction-restriction fragment length polymorphism (PCR- R...

Background: Cellular tolerance to heat stress is mediated by shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response stress. Among different families of these proteins, HSP70 considered be related the development temperature tolerance. Unraveling polymorphism protein genes could a step towards identification genetic markers for selecting heat-tolerant cattle....

In this study the genetic polymorphism of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH genewas amplified by polymerase chain reaction (PCR). PCR products were analyzed using single strand conformation polymorphism (...

The article presents the results of complex research that continues previous work aimed at determining polymorphism CSN2, PRL, LEP, and TNF-α loci analyzing productivity dairy cattle with different genotypes for these loci. goal is to analyze selection populations cows based on typing individuals allelic variants loci, which are associated economically valuable traits but not directly evaluated...

OBJECTIVE To identify a biomarker profile associated with tumor response to chemoradiation (CRT) in locally advanced rectal cancer. BACKGROUND Rectal cancer response to neoadjuvant CRT is variable. Whereas some patients have a minimal response, others achieve a pathologic complete response (pCR) and have no viable cancer cells in their surgical specimens. Identifying biomarkers of response wi...