In methanogenic Archaea, the final step of methanogenesis generates methane and a heterodisulfide of coenzyme M and coenzyme B (CoM-S-S-CoB). Reduction of this heterodisulfide by heterodisulfide reductase to regenerate HS-CoM and HS-CoB is an exergonic process. Thauer et al. [Thauer, et al. 2008 Nat Rev Microbiol 6:579-591] recently suggested that in hydrogenotrophic methanogens the energy of h...

The incorporation in vitro of formate-C14 into normal breast tissue protein, taken from different individuals and from the same individual at different times, was rela tively consistent. Administration of estradiol did not appreciably affect the rate of in corporation of formate-C14 into proteins or protein-bound serine of normal breast tis sue. However, surviving neoplastic breast tissue showe...

Two anaerobic microorgaisms were isolated by the enrichment culture technique from a sodium formate-mineral salts medium inoculated with black mud from the San Francisco Bay. One of these, a motile coccus designated Methanococcus vannielii, carries out a methane fermentation of formate (Stadtman and Barker, 1951). The second organism that was almost equally numerous in the enrichment cultures i...

Mycobacterium vaccae 10 growing in methanol medium synthesizes two inducible alternative NAD(+)-dependent formate dehydrogenases (FDH). In the presence of molybdenum, the dominating form of the enzyme is FDHI with Mr 440 kDa and Km 0.32 mM for sodium formate. FDHI reduced ferricyanide as well as NAD+, and it was reversibly inactivated by formate. NAD+ stabilized FDHI against this inactivation. ...

FocA belongs to the formate-nitrate transporter family and plays an essential role in the export and uptake of formate in organisms. According to the available crystal structures, the N-terminal residues of FocA are structurally featureless at physiological conditions but at reduced pH form helices to harbor the cytoplasmic entrance of the substrate permeation pathway, which apparently explains...

Incidents associated with methanol intoxication resulting from the consumption of fake wine occur not infrequently worldwide. Certain individuals are made blind due to methanol poisoning. The present study aimed to investigate the effects of sodium formate exposure on photoreceptor cells (661W cells). The 661W cells were exposed to sodium formate for 6‑24 h and cell viability was determined usi...

The objective of this study was to determine the optimal inclusion rate of dietary formic acid-ammonium formate (composition by weight was 62% formic acid and 37% ammonium formate) in nursery and grower-finisher diets or grower-finisher diets only. At weaning (d 21 +/- 2), 224 pigs (equal numbers of gilts and barrows) were blocked by BW within sex (28 pigs per BW block, 4 pigs per pen) and assi...

Serpentinization reactions produce highly reduced waters that have hyperalkaline pH and can high concentrations of H 2 CH 4 . Putatively autotrophic methanogenic archaea been identified in the subsurface Samail Ophiolite, Sultanate Oman, though strategies to overcome dissolved inorganic carbon limitation remain be fully understood. Here, we recovered metagenome assembled genomes (MAGs) applied ...