PURPOSE The purposes of this study were to determine whether entactin/nidogen (E/N) is synthesized and secreted by corneal endothelial cells and to characterize the distribution of E/N in the human cornea. METHODS Cultured bovine corneal endothelial cells were metabolically labeled with [35S]methionine. Newly synthesized E/N was detected in cell lysates and culture medium by immunoprecipitati...

Cation concentration and water content were determined in the normal rainbow trout cornea, aqueous humor, lens, and vitreous humor. Anatomically the rainbow trout cornea possesses a thicker epithelium and a more prominent Bowman's membrane than those found in mammalian corneas. Descemet's membrane and the endothelium appear more primitive than their mammalian counterparts. In vitro, the cornea ...

purpose: to compare clear corneal incision and scleral tunnel incision in terms of intraocular pressure (iop), post-operative inflammation, induced astigmatism, and corneal edema in phacoemulcification. methods: this non-controlled clinical trial was conducted on 78 eyes with senile cataract. a clear corneal incision was made in 40 eyes and a scleral tunnel incision was made in 38 eyes. all eye...

purpose: to image leukocyte adherence to the vascular endothelium and extravasation into surrounding tissues in neovascularized corneas using acridine orange, a selective dna stain, in rats methods: corneal neovascularization was induced by placing two sutures in the cornea of one eye of 12 long-evans rats. one week later, acridine orange (4 mg/kg) was injected via the tail vein and the eyes we...

Harlequin ichthyosis is a rare congenital autosomal recessive disorder that causes hyperkeratosis or plate-like keratosis. Hyperkeratosis affects both upper and lower eyelids defective eyelids. Lagophthalmos persistent dry eye will cause desiccation of the cornea, possibly leading to complications such as ectropion, cornea ulceration, corneal perforation, etc. requires regular ocular review pre...

purpose : acanthamoeba keratitis (ak) is a sight-threatening corneal infection with a rapidly increased incidence since 1990s along with the growing popularity of contact lenses. in this study we aimed to study patients with ak and its associated risk factors in farabi eye hospital of tehran, focusing on those with more severe corneal involvement. methods : patients with clinical or laboratory ...

stem cells located at the limbus ,  are the ultimate source for regeneration of the corneal epithelium in the normal and traumatized states.  when limbal stem cells are dysfunctional or deficient, limbal stem cell deficiency (lscd) develops. its surgical management depends on laterality and severity of corneal-limbal involvement. conventional methods of stem cell transplantation are conjunctiva...

background and objective: to determine the diagnostic accuracy and pitfalls of frozen section in ovarian tumors in one of the largest university affiliated gynecologic oncology centers in tehran, and determine the cause of discrepancies. materials and methods: we retrospectively analyzed the results of frozen section and permanent diagnoses of ovarian masses by reviewing the reports in the dep...

Human cornea is the anterior transparent layer of the eye globe. It is an avascular tissue that is directly exposed to the external environment. Cornea focuses the surrounding image by refracting the light through a crystalline lens to the retina. Therefore, its clarity should be well maintained for optimal visibility [1]. Cornea is a multilayered tissue and each layer has its own specificity. ...

Permeation of a drug through the cornea may be studied in vitro by using either the excised cornea of slaughtered animals or an organotypic cornea construct [1]. To compare drug permeability data reported in the literature for excised porcine cornea [2] with those from cornea constructs, the cultivation of an organotypic porcine cornea is required. The porcine cornea consists of three major cel...