In photosystem I from plants and cyanobacteria a phylloquinone molecule, called A1, functions as the secondary electron acceptor. In cyanobacteria, genes that encode for proteins involved in phylloquinone biosynthesis can be deleted. Here, we have studied three different gene deletion mutants called menB, menD, and menE mutants. In these mutants, plastoquinone-9 occupies the A1 binding site. Us...

calcium fluoride nanoparticles with special amount of cerium ion (%1) were synthesized by coprecipitation. fourier transforms infrared spectroscopy (ftir), x-ray diffraction (xrd), absorption and photoluminescence (pl) from caf2ce nanoparticles has been investigated. ftir spectra reveal that there are some amounts of oh, no3 and other organic functional groups on the particle surfaces before th...

Toxicity bioassays are important tools to determine biological effects of chemical agents on species. The questions remained on, what effects have been imposed on each of the different molecular site of cells by chemical exposure and how to find a pattern for chemical toxicity. To address the questions, HepG2 cell lines were exposed to the different concentrations of cisplatin for 24 hours to r...

miconazole is an imidazole antifungal agent, commonly applied topically to the skin or mucous membranes. the aim of this study was to examine the alternative method for gaining mechanism or the bimolecular changes caused by the possible teratogenic effects of miconazole on mice fetus brain tissue using ftir-microspectroscopy. the mice were injected with miconazole (60 mg/kg) on gestation day 9....

Comprehensive spectroscopic characterization of interior and exterior chips of the lherzolitic shergottite Y-984028 has been performed using results from six techniques. Data from UVevisibleenear-IR reflectance spectra, thermal (mid-IR) emission spectra, attenuated total reflectance (ATR) spectra, transmission FTIR spectra, Raman microprobe spectra, and Mössbauer spectra of whole rock and miner...

Protein-bound water molecules play crucial roles in their structure and function, but their detection is an experimental challenge, particularly in aqueous solution at room temperature. By applying attenuated total reflection (ATR) Fourier-transform infrared (FTIR) spectroscopy to a light-driven Cl(-) pump pharaonis halorhodopsin (pHR), here we detected an O-H stretching vibration of protein-bo...

Surface functional group chemistry of intact Gram-positive and Gram-negative bacterial cells and their isolated cell walls was examined as a function of pH, growth phase, and growth media (for intact cells only) using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Infrared spectra of aqueous model organic molecules, representatives of the common functional grou...

This study aimed at examining effective sample treatments and spectral processing for an alternate method of soil nitrate determination using the attenuated total reflectance (ATR) of Fourier transform infrared (FTIR) spectroscopy. Prior to FTIR measurements, soil samples were prepared as paste to enhance adhesion between the ATR crystal and sample. The similar nitrate peak heights of soil past...

The structure of DNA within CLDCs used for gene delivery is controversial. Previous studies using CD have been interpreted to indicate that the DNA is converted from normal B to C form in complexes. This investigation reexamines this interpretation using CD of model complexes, FTIR as well as Raman spectroscopy and molecular dynamics simulations to address this issue. CD spectra of supercoiled ...

evaluation of pre-fixed biological tissues preparation methods for atr-ftir biospectroscopy fourier transform infrared (ftir) spectroscopy in atr (attenuated total reflection) mode is a powerful tool for studying biomedical samples, which can provide important structural information on the molecular composition. currently, formalin fixation and paraffin preservation (ffpp) is the preferred source ...