by oncogenic viruses [simian virus 40 (SV40) or jx)lyoma virus or sequential infection with both viruses], their susceptibility to changes caused by L-fucose was greatly reduced. Thus, virustransformed cells grown in the presence of L-fucose showed much less of a change in the morphology of individual cells, their pattern of association, their rate of growth, and incorporation of protein and RN...

The present study was aimed at verifying whether serum L-fucose, total sialic acid (TSA) and ceruloplasmin can be used as prognostic indicators for type II diabetes mellitus, which will help in detecting complications of diabetes at an earlier stage. Thirty confirmed type II diabetic patients and 30 non-diabetic subjects were considered for the study. Serum L-fucose, total sialic acid and cerul...

The incorporation of fucose-(3)H in rat thyroid follicles was studied by radioautography in the light and electron microscopes to determine the site of fucose incorporation into the carbohydrate side chains of thyroglobulin, and to follow the migration of thyroglobulin once it had been labeled with fucose-(3)H. Radioautographs were examined quantitatively in vivo at several times after injectio...

Four wheat germ agglutiniti (WGA)-resistant variants of the highly metastatic murine tumor MDAY-D2 were found to have altered tumorigenic and metastatic capacities in the syngeneic DBA/2 host. The parental tumor metastasized to lungs (100%) and completely replaced the liver (100%) in the syngeneic DBA/2 host. In contrast, the variants formed large, discrete tumor nodules in the liver (100%) and...

A DPN+-dependent L-fucose dehydrogenase has been isolated from pork liver and purified over 300-fold by a combination of ammonium sulfate fractionation, diethylaminoethyl cellulose chromatography, gel filtration on Sephadex G-100, and preparative polyacrylamide gel electrophoresis. The pH optimum for the dehydrogenase was 8.7 and K,,, values were 2.0 x 10d5 M for DPN+ and 3.2 x 10m4 M for L-fuc...

To survive in its sand fly vector, the trypanosomatid protozoan parasite Leishmania first attaches to the midgut to avoid excretion, but eventually it must detach for transmission by the next bite. In Leishmania major strain Friedlin, this is controlled by modifications of the stage-specific adhesin lipophosphoglycan (LPG). During differentiation to infective metacyclics, d-arabinopyranose (d-A...

The first amperometric biosensing method for the determination of L-fucose is described. L-Fucose is the objective of much current research, as it is considered as a potential marker for various pathologic disorders. Recombinant L-fucose dehydrogenase, having as cofactor beta-nicotinamide adenine dinucleotide phosphate (NAD+P), was cross-linked in a water-soluble photosensitive polymer matrix, ...

Mammalian Notch receptors require modification by fucose on epidermal growth factor-like (EGF) repeats of their extracellular domain to respond optimally to signal induction by canonical Notch ligands. Inactivation of the Golgi GDP-fucose transporter Slc35c1 in mouse or human does not cause marked defects in Notch signaling during development, and shows milder fucosylation defects than those ob...

Carbohydrate-protein interactions are known to be important in gamete interactions. Several evidence indicated that a fucose-containing sulfated polysaccharide fucoidan was potential inhibitor of fertilization in vitro and thus fucose seemed to be part of the recognition signal of gamete interaction in mammals. In recent investigation we found that α-L-fucosidase activity was present in boar sp...

Alpha-L-fucose is a 6-carbon deoxyhexose that is commonly incorporated into human glycoproteins and glycolipids. It is found at the terminal or preterminal positions of many cell-surface oligosaccharide ligands that mediate cell-recognition and adhesion-signaling pathways. These include such normal events as early embryologic development and blood group recognition and pathologic processes incl...