The primary structure of the membranous segment of rabbit liver cytochrome b5 has been determined to be as follows: (Formula: see text), New methods were developed for the separation of peptide fragments. These methods, which are particularly useful for work with hydrophobic peptides, involved gel permeation chromatography and reverse phase high performance liquid chromatography (HPLC) using et...

Submit Manuscript | http://medcraveonline.com Abbreviations: GLPs: Gracilaria Lemaneiformis Polysaccharides; FTIR: Fourier Transform Infrared Spectroscopy; GC: Gas Chromatography; HPGPC: High Performance Gel Permeation Chromatography; NK: Natural Killer; IL-2: Interleukin-2; IFN-γ: Interferon-γ; TNF-α: Tumor Necrosis Factor-α; BRM: Biological Response Modifier; ConA: Concanavalin A; LPS: Lipo P...

The exopolysaccharide (EPS) separated from the entomopathogenic fungus Metarhizium anisopliae was determined by gel permeation chromatography to be homogeneous. The high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAE-PAD) showed a content of monosaccharides D-galactosamine and D-fucose at a molar ratio of about 2:1. The results obtained from Fourier transform...

Polyvinyl benzyl chloride (PVBC) was synthesized by free radical polymerization of 4-vinylbenzyl using benzoyl peroxide initiator at 60 °C. PVBC synthesised in different solvents such as toluene, xylene, 1,4-dioxane, and tetrahydrofuran. The polymers were structurally characterized 1H NMR FTIR spectroscopic techniques. thermal property the polymer investigated thermogravimetric analysis (TGA) d...

BACKGROUND We present the case of an 80-year-old woman who was admitted to hospital with an intermittent volvulus of the right colon. A total colectomy was performed. Initially, serum amylase and lipase increased concordantly, but after a few weeks amylase normalized (85 U/L), whereas lipase increased to 3764 U/L. This discrepancy and persistence of hyperlipasemia suggested a macromolecular for...

The apoprotein of human serum beta lipoprotein has been prepared in a lipid-free and soluble form by a technique of interfacial extraction in the presence of guanidinium hydrochloride and nonionic amphiphile. Gel permeation chromatography of maleylated apobeta lipoprotein separates two protein fractions with different amino acid compositions. The smaller particle has a molecular weight of 2.6 x...

A polysaccharide separated from Paecilomyces sp. was determined by gel permeation chromatography to be homogeneous. HPLC showed a monosaccharide containing D-glucose and D-fructose at a ratio of about 2:1. The results obtained from IR, 1H NMR, and 13C NMR analyses confirmed the proposed structure.

METHODS. The human lenses were diabetic or age-matched control lenses from donors 57, 59, 69, and 72 years of age. Lenses were also obtained from streptozotocin-induced diabetic rats. Individual lens crystallins in water-soluble fractions were separated by gel-permeation chromatography. The high ( H)and low ( L)-molecular-weight fractions were analyzed by electrospray ionization mass spectrometry.

A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enteroto...