Cwh6 is a temperature-sensitive cell wall mutant of Saccharomyces cerevisiae. CWH6 was found to be identical to SPT14, a gene that is highly homologous to both human PIG-A and to RFAK from Salmonella typhimurium. PIG-A and RFAK are involved in transferring N-acetylglucosamine to, respectively, a GPI anchor precursor and to lipopolysaccharides. Because cell walls of cwh6 are greatly reduced in m...

We have developed a method for transferring exogenous DNA molecules into isolated mammalian mitochondria using bacterial conjugation. In general, we accomplish this by (i) inserting an origin of DNA transfer (oriT) sequence into a DNA construct, (ii) transforming the construct into an appropriate Escherichia coli strain and then (iii) introducing the mobilizable DNA into mitochondria through co...

The question of targeted control over trajectories systems differential equations encountered in the theory genetic and neural networks is considered. Examples are given transferring corresponding to network states from basin attraction one attractor target attractor. This article considers a system ordinary that arises gene networks. Each trajectory describes current future network. possibilit...

To evaluate the potentiality of applying gene therapy to endotoxemia in high-risk patients, we investigated the effects of transferring an adeno-associated virus serotype 2 (AAV2)-mediated BPI-Fcgamma1 gene on protecting mice from challenge of lethal endotoxin. The chimeric BPI-Fcgamma1 gene consists of two parts, one encods functional N-terminus (1 to 199 amino acidic residues) of human BPI, w...

α-Tocopherol transfer protein (α-TTP) is a ~32 kDa protein expressed mainly in hepatocytes. The major function of the protein is to bind specifically to α-tocopherol and, together, the complex transfers from late lysosomes to the cell membrane. A previous study indicated that some factors might be required in the transferring process. However, there is little information available about the pot...

Trispecific microcell hybrids were prepared by transferring limited numbers of chromosomes from a human/mouse gene-transfer cell line to a Chinese hamster recipient line. The donor cells employed were murine L-cells that stably expressed the human form of the enzyme hypoxanthine phosphoribosyltransferase. Karyotypic, zymographic, and back-selection tests of the resulting human/mouse/Chinese ham...

Leukodystrophies (LDs) refer to a group on inherited diseases in which molecular abnormalities of glial cells are responsible for exclusive or predominant defects in myelin formation and/or maintenance within the central and, sometimes, the peripheral nervous system. For three of them [X-linked adrenoleukodystrophy (X-ALD), metachromatic (MLD) and globoid cell LDs], a gene therapy strategy aimi...

Impaired control of the complement system activation due to mutations in complement factor H (CFH) has been described in two apparently unrelated human diseases, membranoproliferative glomerulonephitis type II (MPGN2) and non-Shiga toxin-associated haemolytic uraemic syndrome (non-Stx-HUS). Mouse models of these diseases have been developed by Pickering et al., by knocking-out Cfh gene (MPGN2) ...