Epidemiological findings have indicated that red meat increases the likelihood of colorectal cancer. Aim of this study was to investigate whether hemoglobin, or its prosthetic group heme, in red meat, is a genotoxic risk factor for cancer. Human colon tumor cells (HT29 clone 19A) and primary colonocytes were incubated with hemoglobin/hemin and DNA damage was investigated using the comet assay. ...

BACKGROUND The aim of this study was to analyze whether in chronic lymphocytic leukemia the cytosolic release of histone H1.2, a new apoptogenic mechanism induced by DNA damage, was associated with the presence of genetic abnormalities and with the response to treatment. DESIGN AND METHODS Primary tumoral chronic lymphocytic leukemia cells from 25 patients were investigated for histone H1.2 c...

A new point of view for genetic damage assessment using the comet assay is proposed based on number migration groups, comets in each group, and groups with highest comets. Human lymphocytes were exposed to different concentrations Methyl Methane Sulfonate (MMS), Maleic Hydrazide (MH), 2,4-Dichlorophenoxyacetic (2,4-D), N-nitroso diethylamine (NDEA). Using assay, means determined later grouped a...

Liver is the main organ of xenobiotic biotransformation. Since biotransformation may generate highly mutagenic active metabolites and reactive oxygen species, liver cells are both the producers and targets for these compounds. Xenobiotics can lead to liver tumour formation via genotoxic or non-genotoxic mechanisms. Non-genotoxic chemicals are often inducers of monooxygenase reactions depending ...

Cellular senescence suppresses cancer by arresting cell proliferation, essentially permanently, in response to oncogenic stimuli, including genotoxic stress. We modified the use of antibody arrays to provide a quantitative assessment of factors secreted by senescent cells. We show that human cells induced to senesce by genotoxic stress secrete myriad factors associated with inflammation and mal...

Application of exogenic bioactive natural plant compounds with protective, anti-mutagenic and anti-genotoxic effects against various chemical and physical agents is one of the modern approaches for reduction of the mutagenic burden of cells. Objective: The present study aims to provide data on the cytoxic and genotoxic effects of Papaver rhoeas L. water leaf extract and its anti-cytotoxic and a...