The promise of whole genome amplification (WGA) is that genomic DNA (gDNA) quantity will not limit molecular genetic analyses. Multiple displacement amplification (MDA) and the OmniPlex PCR-based WGA protocols were evaluated using 4 and 5 ng of input gDNA from 60 gDNA samples from three tissue sources (mouthwash, buffy coat, and lymphoblast). WGA DNA (wgaDNA) yield and genotyping performance we...

OBJECTIVE Identifying genotyping errors is an important issue in genetic research, yet it has been relatively less studied in samples consisting of unrelated individuals. In this article, we consider several models of genotyping errors, which were originally proposed for pedigree data, for unrelated population samples with single nucleotide polymorphism (SNP) genotype data. The mathematical con...

Whole genome amplification can faithfully amplify genomic DNA (gDNA) with minimal bias and substantial genome coverage. Whole genome amplified DNA (wgaDNA) has been tested to be workable for high-throughput genotyping arrays. However, issues about whether wgaDNA would decrease genotyping performance at increasing multiplexing levels and whether the storage period of wgaDNA would reduce genotypi...

BACKGROUND Hemolytic disease of the fetus and newborn, classically caused by maternal-fetal incompatibility of the Rh blood group D antigen, can be prevented by RhIG prophylaxis. While prophylactic practices for pregnant women with serologic weak D phenotypes vary widely, RHD genotyping could provide clear guidance for management. This analysis evaluated the financial implications of using RHD ...

the growing number of immunocompromised individuals has increased the incidence of infections caused by candida species during the recent decades. typing of c. albicans on the basis of dna sequences at multiple loci has greatly advanced our knowledge about the epidemiology and phylogeny of candidiasis. the aim of this study was to evaluate the diversity, and genetic relationships among c. albic...

the objective of this study was to determine the prevalence and distribution of chlamydia trachomatisgenotypes in shiraz, iran. two hundred twelve cervical swab samples were collected from women attendingshahid motahari polyclinic in shiraz, iran. the endocervical specimens were screened for c. trachomatis byplasmid pcr. genotyping was performed in c. trachomatis-positive samples by nested pcr ...

conclusions the results of the current study demonstrated that type iii is the predominant type in hamedan, followed by types v, type ii, type ia, ib and iv, respectively. use of the molecular serotyping (ms) method such as pcr assay as an alternative to conventional serotyping (cs) method leads to accurate, sensitive, specific, and fast typing of gbs isolates. results among the 62 gbs isolates...

background: infectious bronchitis virus (ibv) is the causative agent of avian infectious bronchitis, which is characterized by respiratory, reproductive, and renal signs. ibv is a highly variable virus with a large number of genotypes. s1 gene sequencing has been used for molecular epidemiological studies and genotypic characterization of ibv. objective: to better understand the molecular epide...

conclusions the c genotype should be considered as the prevalent ev71 circulating genotype in iran, particularly in cases of aseptic meningitis. patients and methods viral rna was extracted from 170 cerebrospinal fluid samples obtained from children aged under 8 years with a primary diagnosis of aseptic meningitis. specific ev71 pcr was conducted to identify the genotype of the detected ev71 vi...