Direct ESI-MS of proteins in nonvolatile buffer requires the generation very fine initial droplets to resolve peak for meaningful analysis. This is usually achieved using nanoESI capillary with an inner diameter ∼1 μm or smaller. Recently we reported “true nanoelectrospray” from a micropipette tip large under super-atmospheric pressure condition (J. Am. Soc. Mass Spectrom. 2022, 33, 491–498). T...

PURPOSE To present a new proposal to evaluate the healing of an open subcutaneous and skin wound, which we termed "volumetry". METHODS A total of 32 circular wounds were performed in the subcutaneous tissues and skin of four feet of pigs (8 each). Each wound had about 1 cm in diameter and was 0.2 cm deep. Volume was calculated from the wound filled with saline and mass Xantopren. With the aid...

A glass capillary ultramicroelectrode (tip diameter approximately 1.2 microm) having an electrokinetic sampling ability is described. It is composed of a pulled glass capillary filled with an inner solution and three internal electrodes (Pt working and counter electrodes and an Ag/AgCl reference electrode). The voltammetric response of the capillary electrode is based on electrokinetic transpor...

Volcanic ash particles can be remelted by the high temperatures induced in volcanic lightning discharges. The molten particles can round under surface tension then quench to produce glass spheres. Melting and rounding timescales for volcanic materials are strongly dependent on heating duration and peak temperature and are shorter for small particles than for large particles. Therefore, the size...

Electrospray ionization (ESI) has revolutionized mass spectrometry (MS), providing a facile method for the ionization of macromolecules for analysis by mass. The development of nanoESI-MS has further extended the utility of ESI-MS, permitting the analysis of small-volume samples with enhanced sensitivity over conventional ESI-MS. Traditional nanoESI-MS experiments use pulled-glass capillary emi...

This paper describes a microfluidic multi-injector (MMI) that can generate temporal and spatial concentration gradients of soluble molecules. Compared to conventional glass micropipette-based methods that generate a single gradient, the MMI exploits microfluidic integration and actuation of multiple pulsatile injectors to generate arbitrary overlapping gradients that have not previously been po...

In this paper a novel capillary electrophoresis chip (CEC) is presented with integrated platinum electrodes and simplified conductivity detector. CEC is fabricated by the method of mechanical modification with probe on organic glass. Capillary electrophoresis chip can rapidly completed ion separation by simulation of concentration distribution and zonebroadening. Detection circuit is simple whi...

An efficient photochemical method for the site-specific immobilization and patterning of (bio)molecules inside glass capillary tubes is reported. The strategy involves the photodeprotection of reactive aminooxy groups on surfaces and subsequent reaction with aldehyde containing (bio)molecules.

In this work a glass micromodel which its grains and pores are non-uniform in size, shape and distribution is considered as porous medium. A two-dimensional random network model of micromodel with non-uniform pores has been constructed. The non-uniformity of porous model is achieved by assigning parametric distribution functions to pores throat and pores length, which was measured using ima...