Serum globulin from donor sheep made nephritic by immunization with glomerular basement membrane and subsequently nephrectomized contained specific kidney-fixing antibody and was capable of inducing an immediate, although transient, glomerulonephritis when injected into unilaterally nephrectomized lambs. This nephritis was characterized by immediate proteinuria, PMN infiltration into the glomer...

Cultured rat glomerular epithelial cells (GEC) were examined for their ability to release extracellular matrix-degrading proteinases with [3H]gelatin as substrate. GEC-conditioned media, under serum-free conditions, contained modest amounts of gelatinase activity (1 to 10 U/mg of protein); the activity was maximal at neutral pH, was inhibited by zinc chelators, was not inhibited by tissue inhib...

Ferritin-conjugated antibody has been used to identify by electron microscopy the sites at which nephrotoxic globulins localize in rat kidney during acute experimental glomerulonephritis. Antibody was concentrated in the glomerular basement membrane and in basement membrane-like material contained in distended cisternae of the endoplasmic reticulum. These data confirm and amplify, at the ultras...

BACKGROUND Anti-glomerular basement membrane (anti-GBM) disease classically presents with aggressive necrotizing and crescentic glomerulonephritis, often with pulmonary hemorrhage. The pathologic hallmark is linear staining of GBMs for deposited immunoglobulin G (IgG), usually accompanied by serum autoantibodies to the collagen IV alpha-3 constituents of GBMs. METHODS Renal pathology files we...

The glomerular basement membrane (GBM) is thin extracellular matrices which separate the visceral epithelium of the glomerulus from the capillary endothelium and serves as a filter for blood molecules. GBMs are composed of type IV collagen, laminin, heparan sulfate proteoglycan, entactin/nidogen, fibronectin and other minor glycoproteins which are not characterized. Recent technological advance...

Title:Comparison of double filtration plasmapheresis with immunoadsorption therapy in patients with anti-glomerular basement membrane nephritis Title: Comparison of double filtration plasmapheresis with immunoadsorption therapy in patients with anti-glomerular basement membrane nephritis After reading through your manuscript, we feel that the quality of written English needs to be improved befo...

Collagen IV is a major component of basement membranes, and mutations in COL4A1, which encodes collagen IV alpha chain 1, cause a multisystemic disease encompassing cerebrovascular, eye and kidney defects. However, COL4A1 renal disease remains poorly characterized and its pathomolecular mechanisms are unknown. We show that Col4a1 mutations in mice cause hypotension and renal disease, including ...

Specific antibodies to laminin, type IV collagen, basement-membrane proteoglycan, and fibronectin have been used in immunofluorescence microscopy to study the development of basement membranes of the embryonic kidney. Kidney tubules are known to form from the nephrogenic mesenchyme as a result of an inductive tissue interaction. This involves a change in the composition of the extracellular mat...