Glucocorticoid treatment dramatically inhibits growth of the wild-type DDT1MF-2 hamster smooth muscle tumor cell line (DDT-WT) but not that of a glucocorticoid-selected clonal variant (DDT-GR). Our objective was to further define the level of glucocorticoid resistance in DDT-GR cells. Glucocorticoid receptors were confirmed to be less abundant in DDT-GR cells, but the immunoreactivity and molec...

Enhanced dopamine efflux in the prefrontal cortex is a well-documented response to acute stress. However, the underlying mechanism(s) for this response is unknown. Using in vivo microdialysis, we demonstrate that blocking glucocorticoid receptors locally within the rat prefrontal cortex results in a reduction in stress-evoked dopamine efflux. In contrast, blocking glucocorticoid receptors in th...

There ¡sa range of levels of glucocorticoid receptor numbers seen in the various subclasses of acute lymphatic leukemia (ALL). This variability cannot be explained by the known cor relation between active cell proliferation and an increase in the number of receptors, since the tumors with the highest growth fraction (i.e., Burkitt's lymphoma and T-cell leukemia) tend to have lower average rece...

Lymphoid cells contain specific receptors for glucocorticoids. We have used [3H]dexamethasone-21-mesylate to label covalently glucocorticoid receptors in rat thymic lymphocytes and in neoplastic cells obtained from patients with acute lymphoblastic leukemia and malignant lymphoma. The covalently labeled glucocorticoid receptors were identified by polyacrylamide gel electrophoresis (in the prese...

Dexamethasone binding to both rabbit iris-ciliary body and rabbit liver glucocorticoid receptors was compared by means of a batch assay in which the binding components were adsorbed to hydroxylapatite. The results showed that homogenates of both the rabbit iris-ciliary body and the rabbit liver contained a single class of high-affinity receptors. Binding affinity was virtually identical for the...

3H-triamcinolone acetonide labeled glucocorticoid receptors in normal lymphoid tissues can be resolved into two component by DEAE chromatography: peak I elutes at 0.04 M salt and peak II is 0.22 M salt. By glycerol gradient centrifugation, peak I is 3.5S and peak II 8.5S. Peak I binds to DNA and chromatin, while peak II binds to neither. After heat activation, peak II alters its coefficient of ...

UNLABELLED Stress and glucocorticoids stimulate the rapid mobilization of endocannabinoids in the basolateral amygdala (BLA). Cannabinoid receptors in the BLA contribute to anxiogenesis and fear-memory formation. We tested for rapid glucocorticoid-induced endocannabinoid regulation of synaptic inhibition in the rat BLA. Glucocorticoid application to amygdala slices elicited a rapid, nonreversib...