Gene sequences annotated as proteins of unknown or non-specific function and hypothetical proteins account for a large fraction of most genomes. In the strictly anaerobic and organohalide respiring Dehalococcoides mccartyi, this lack of annotation plagues almost half the genome. Using a combination of bioinformatics analyses and genome-wide metabolic modelling, new or more specific annotations ...

Linkage relationships of nine enzyme loci; aconitase (Acon ), esterase (Est), glucosephosphate isomerase A and B ( Gpi), glycerate-2-dehydrogenase (G2dh), malic enzyme (Me ), phosphoglycerate kinase (Pgk), phosphoglucomutase (Pgm ) and superoxide dismutase (Sod), were investigated in sunfishes (Lepomis, Centrarchidae). Reciprocal F(1) hybrids produced from crosses between green sunfish (Lepomis...

Spontaneously transformed mouse cell lines heterozygous for electrophoretic markers have been studied to determine the relationship between gene dosage and phenotype. It is shown that a clone with an electrophoretic pattern for glucosephosphate isomerase of three bands in a ratio of 4A:4AB:1B contains three copies of chromosome 7, which carries the gene for this enzyme. A clone from a different...

Eight erythrocyte enzymes were examined for thermostability in an unselected sample of 100 newborn infants. Three thermolabile variants, one each of lactate dehydrogenase, glucosephosphate isomerase, and glucose-6-phosphate dehydrogenase, were identified, none of which was detectable as a variant by standard electrophoretic techniques. All were inherited. This frequency of 3.8 heritable thermos...

W report that the kinetic constants for several mutants of ketosteroid isomerase (KSI) from our 2011 publication are incorrect. Jason Schwans and Daniel Herschlag, the paper’s first and corresponding authors, take full responsibility for and deeply regret these errors. Based on extensive control experiments, the errors likely arose from low levels (∼0.1−1%) of contaminating wild-type enzyme int...

Previous work on the phosphoglucose isomerase (PGI) polymorphism of Colias butterflies led to predictions concerning aspects of differential survivorship and fecundity among the polymorphic genotypes in the wild. Explicit assumptions underlying these predictions were that functional differences among genotypes at the in vitro biochemical level reflected roughly corresponding differences in vivo...

In a s t r a i n of K. l a c t i s . the RAG 2 gene (1) i s found as a recessive a l l e l i c form, rap2. The s t ra in cannot grow on glucose if the respi ra tory pathway i s blocked by mitochondrial i n h i b i t o r s . The RAG2 gene has been cloned and i t s nucleot ide sequence determined. The 557 aminoacids long open reading frame has a homology of 56,5% with mouse neuroleukine (2) which...