Background: Hypoglycemic effect of Juglans regia leaves was reported in other previous researches.   Objective: To investigate the mechanism of hypoglycemic action of Julans regia Leaves Methanolic Extract (JRLME), we designed the following study.   Methods: Male wistar rats were classified in five groups, each of six, including alloxan-induced diabetic rats treated with JRLME at doses of 250...

During studies of the nutritional utilization of pyridoxine 5*-b-D-glucoside, a major form of vitamin B6 in plants, we detected two cytosolic b-glucosidases in jejunal mucosa. As expected, one was broad specificity b-glucosidase that hydrolyzed aryl b-D-glycosides but not pyridoxine b-D-glucoside. We also found a previously unknown enzyme, designated pyridoxine-b-D-glucoside hydrolase, that eff...

Digestion of starch in mammals occurs chiefly in the mouth and small intestine through the catalytic activity of ar-amylase and maltase (a-glucosidase). Extensive cw-amylolysis of amylose and amylopectin results in the formation of maltose, glucose, and a mixture of branched oligosaccharides (cr-amylase dextrins) (1). The latter compounds, whose structure is currently being investigated by seve...

The dried rhizome of Belamcanda chinensis is an important Chinese traditional medicine used for the treatment of inflammation and many other disorders. Previously, we reported the hypoand antihyper-glycemic effects of the aqueous leaf extract of B. chinensis (BCL) and identified the isoflavones as its principal active fraction. In the present study, the α-glucosidase inhibitory effect of BCL an...

AIM This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase, at optimum temperature and optimum pH) of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia. MATERIALS AND METHODS To produce enzyme from a single colony of E. cloacae WPL 214, 98 × 10(10) CFU/ml of isola...

BACKGROUND Trichoderma reesei is a widely used industrial strain for cellulase production, but its low yield of β-glucosidase has prevented its industrial value. In the hydrolysis process of cellulolytic residues by T. reesei, a disaccharide known as cellobiose is produced and accumulates, which inhibits further cellulases production. This problem can be solved by adding β-glucosidase, which hy...

The gene encoding for an extremely thermostable oligo-1,6-glucosidase from Bacillus thermoglucosidasius KP1006 (DSM2542, obligate thermophile) was sequenced. The amino acid sequence deduced from the nucleotide sequence of the gene (1686 base pairs) corresponded to a protein of 562 amino acid residues with a Mr of 66,502. Its predicted amino acid composition, Mr, and N-terminal sequence of 12 re...

objective diabetes mellitus is manifested by hyperglycaemia. different treatments such as diets and drugs are recommended for diabetes control. for various reasons in recent years traditional plant (herbal) therapies as prescribed by indigenous systems of medicine with different mechanisms have commonly been used. the digestive enzymes such as alpha glucosidase are among these herbal remedies. ...

Membrane associated alpha-glucosidase activity was investigated in a methanogenic bioreactor (MR) and a biosulfidogenic bioreactor (SR). Temperature and pH optima studies showed temperature optima of 50 degrees C and pH optima of 8.0 for the alpha-glucosidases from both the MR and SR. Sulfide (at a concentration of 150 mg l(-1)) resulted in the complete loss of all alpha-glucosidase activity in...

β-Glucosidase, which hydrolyzes cellobiose into two glucoses, plays an important role in the process of saccharification of the lignocellulosic biomass. In this study, we optimized the activity of β-glucosidase of brown-rot fungus Fomitopsis pinicola KCTC 6208 using the response surface methodology (RSM) with various concentrations of glucose, yeast extract and ascorbic acid, which are the most...