p-Hydroxyphenylacetaldoxime (HPAAO) (anti and syn forms) obtained by fermentation arid its analogs chemically synthesized were tested for their activities to inhibit various glycosidases. HPAAO inhibited bovine liver /3-galactosidase in a competitive manner at pH 17.0 with an apparent Ki value of 8 x 10-8 M. HPAAO also inhibited various mammalian /3.glycosidases which had pH optima between 6.0 ...

The effect of either high-cellulose (15%) or regular-cellulose (5%) diets on fecal bacterial glycosidases was assessed in two groups of ten Wistar rats, each given an injection of 1,2-dimethylhydrazone, and in two groups of six control rats. During a 4-month period, fecal activities of bacterial /3-galactosidase and /3-acetylglucosaminidase were reduced markedly in control rats maintained on th...

Although lysosomal enzyme activities are known to vary in response to numerous physiological and pharmacological stimuli, the relationship between lysosomal enzyme activity and enzyme concentration has not been systematically studied. Therefore we developed radioimmunoassays for two lysosomal glycosidases in order to determine lysosomal enzyme concentration. beta-Galactosidase and beta-glucuron...

Glycosidases are generally glycoproteins from lysosomal origin that catalyze the hydrolysis of glycoproteins, glycolipids and glycosaminoglycans, as well as synthetic substrates. They accomplish a very important role (partially known) in physiological circumstances, and their dysfunction causes pathological processes, where they have been mainly studied in connection to the lysosomal storage di...

Glycosidases are associated with various human diseases. The development of efficient and specific inhibitors may provide powerful tools to modulate their activity. However, achieving high selectivity is a major challenge given that glycosidases with different functions can have similar enzymatic mechanisms and active-site architectures. As an alternative approach to small-chemical compounds, p...

background: glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. although hexosaminidase (hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, glucosidase is considered a marker for epididymal function and azoospermia. objective: the aim of this study was to determine hex activity in seminal pl...