Anchoring of proteins to membranes by glycosylphosphatidylinositols (GPIs) is ubiquitous among all eukaryotes and heavily used by parasitic protozoa. GPI is synthesized and transferred en bloc to form GPI-anchored proteins. The key enzyme in this process is a putative GPI:protein transamidase that would cleave a peptide bond near the COOH terminus of the protein and attach the GPI by an amide l...

This paper introduces a novel sensor concept for surface exploration in robotic applications. We present an IMU-based haptic sensor that accurately estimates the orientation of contact points. One of the biggest advantages of our proposed sensor concept is the extraction of absolute surface orientation without being affected by inaccuracies in the forward kinematics. Conventional sensors requir...

Mapping with uncertainty representation is required in many research domains, especially for localization. Although there are investigations regarding the of pose estimation an ego-robot map information, quality reference maps often neglected. To avoid potential problems caused by errors and a lack quantification, adequate measure required. In this paper, uncertain building models abstract surf...

Glycosyl phosphatidylinositols (GPIs) are used to anchor many proteins to the cell surface membrane and are utilized in all eukaryotic cells. GPI anchoring units are attached to proteins via a transamidase reaction mediated by a GPI transamidase complex. We isolated one of the components of this complex, mGPAA1 (murine GPI anchor attachment), by the signal sequence trap method. mGPAA1 cDNA is a...

Vaccination against the ubiquitous parasite Toxoplasma gondii would provide the most efficient prevention against toxoplasmosis-related congenital, brain and eye diseases in humans. We investigated the immune response elicited by pathogen-specific glycosylphosphatidylinositol (GPI) glycoconjugates using carbohydrate microarrays in a BALB/c mouse model. We further examined the protective propert...

Inhibitors: Causation or Association? To the Editor: Trials of glycoprotein IIb/IIIa inhibitors (GPI) in acute coronary syndromes (ACS) reveal that thrombocytopenia occurs with an incidence of up to 3.7%.1 McClure et al2 confirm the association of thrombocytopenia and ACS and correlate this with adverse bleeding and ischemic events. However, unlike previous studies, they found no association be...

Abstract This research aims to assess a conceptual framework that includes radio frequency identification (RFID) technology usage and integrated with supply chain (SC) global positioning information system (GPIS) as antecedents performance (SCP). A Web‐based survey questionnaire was used for the data collection process. The derived hypotheses were tested by structural equation modeling. Data ga...

Ensuring equal access to affordable higher education for women and men has become a crucial target of the UN’s SDG 5, while gender disparity persists in various systems. This study employed per capita GDP, gross enrollment ratio (GER), parity index (GPI) demonstrate how higher-education systems have expanded, resulting transformation parity. We selected Japan, Korea, Taiwan, UK as research targ...

The African trypanosome, Trypanosoma brucei, has been used as a model to study the biosynthesis of GPI (glycosylphosphatidylinositol) anchors. In mammalian (bloodstream)-form parasites, diacyl-type GPI precursors are remodelled in their lipid moieties before attachment to variant surface glycoproteins. In contrast, the GPI precursors of insect (procyclic)-form parasites, consisting of lyso-(acy...

Homologues of Gpi8p, Gaa1p, Gpi16p, Gpi17p, and Cdc91p are essential components of the GPI transamidase complex that adds glycosylphosphatidylinositols (GPIs 1) to newly synthesized proteins in the ER. In mammalian cells, these five subunits remain stably associated with each other in detergent. In yeast, we find no stable stoichiometric association of Gpi17p with the Gpi8p-Gpi16p-Gaa1p core in...