The glutathione peroxidase-glutathione reductase system, an alternative pathway for metabolic utilization of H2O2 [Chance, Sies & Boveris (1979) Physiol. Rev. 59, 527-605], was investigated in Trypanosoma cruzi, an organism lacking catalase and deficient in peroxidase [Boveris & Stoppani (1977) Experientia 33, 1306-1308]. The presence of glutathione (4.9 +/- 0.7 nmol of reduced glutathione/10(8...

Wild-type recombinant horseradish peroxidase purified and refolded from Escherichia coli inclusion bodies has been studied in the system of bis(2-ethylhexyl)sulphosuccinate sodium salt (Aerosol OT)-reversed micelles in octane. In contrast with native horseradish peroxidase the wild-type recombinant enzyme forms dimeric structures as judged by sedimentation analysis. Peroxidase substrates affect...

Rare earth elements have been tested in control of plant diseases. Lanthanum (La) was the Alternaria solani (in vitro) and tomato early blight vivo) using concentration 0; 0.1; 0.2; 0.4 0.8 g L-1. In vitro, were diluted V8 culture medium evaluated for mycelial growth rate index (MGRI) pathogen sporulation. vivo, 24 hours after application inoculate inoculation, leaflets collected quantification...

Application of nanotechnology is crucial for a sustainable increase in food production to cope with the increasing demand burgeoning population. Wheat has significantly security Pakistan help nanotechnology. In biological systems, utilization nanoparticles been increased due their growth-promoting effects on germination, photosynthetic attributes, nutrient use efficiency and metabolic activitie...

Aluminum toxicity is one of the most deleterious factors for plant growth in acidic soils because over 50% of the world’s potentially arable lands are acidic. In recent years, considerable research has been conducted to understand the mechanisms of Al toxicity and tolerance in plants. This paper reviews the effects of different concentration of Al on plant shoot, root physiological parameters s...

Previously reported rates of reaction between six mutant strains of the enzyme horseradish peroxidase (HRP) and a test substrate, 2-methoxyphenol, were found to correlate with characteristic binding distances computed using molecular simulation. The correlation (R(2) = 0.86) bears out a working hypothesis that, based on a quantitative structure-activity relationship (QSAR) we had previously dev...

The spectroscopic and kinetic characterization of two intermediates from the H₂O₂ oxidation of three dimethyl ester [(proto), (meso), (deuteroporphyrinato) (picdien)]Fe(III) complexes ([FePPPic], [FeMPPic] and [FeDPPic], respectively) pinch-porphyrin peroxidase enzyme models, with s = 5/2 and 3/2 Fe(III) quantum mixed spin (qms) ground states is described herein. The kinetic study by UV/Vis at ...

A chemiluminescence method was developed to measure thyroid peroxidase (TPO) activity and the inhibitory effect of anti-TPO antibodies in purified porcine TPO. The TPO preparation was characterized kinetically and controlled by Western-blotting technique. The chemiluminescence method proved to be reproducible and much more sensitive than the widely used guaiacol method, being able to detect TPO...