To reveal the full potential of human pluripotent stem cells, new methods for rapid, site-specific genomic engineering are needed. Here, we describe a system for precise genetic modification of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). We identified a novel human locus, H11, located in a safe, intergenic, transcriptionally active region of chromosome 22, as t...

In summer 2013, an excess of paediatric cases of haemolytic uraemic syndrome (HUS) in a southern region of Italy prompted the investigation of a community-wide outbreak of Shiga toxin 2-producing Escherichia coli (STEC) O26:H11 infections. Case finding was based on testing patients with HUS or bloody diarrhoea for STEC infection by microbiological and serological methods. A case-control study w...

Laser Powder Bed Fusion (LPBF) printing is used for manufacturing conformal cooling injection molds with internal channels. Currently, the steel to manufacture such by LPBF maraging 18Ni300. However, this softer at high temperatures and less resistant corrosion than reference steel, AISI H11. Moreover, 18Ni300 contains cobalt, an element that can be harmful health of people handling powders mad...

Grid integration of Renewable Energy Systems (RES) involves various types power electronics-based converters and inverters. The use these electronic-based devices results in inducing both current voltage harmonics to the grid. In order reduce harmonics, especially for large-scale RES, harmonic forecasting is one many techniques used design mitigation devices. core objective this work develop a ...

In common wheat (Triticum aestivum L.), allelic variations of Glu-A1 locus have important influences on grain end-use quality. Among the three Glu-A1 alleles, Glu-A1a and -A1b encode the high-molecular-weight glutenin subunits (HMW-GSs) 1Ax1 and 1Ax2*, respectively, whereas Glu-A1c does not specify any subunit. Here, we detected a total of 11 Glu-A1 locus haplotypes (H1 to H11) in three wheat s...

BACKGROUND The increasing incidence of multidrug-resistant Gram negatives, such as carbapenem-resistant Enterobacteriaceae (CRE), has resulted in a critical need for new antimicrobials. Most studies of new antimicrobials have been performed in patients with nondrug-resistant pathogens. We performed a retrospective analysis of patients with CRE infections to inform the design of phase 3 clinical...

BACKGROUND The increasing incidence of carbapenem-resistant Enterobacteriaceae (CRE), has resulted in a difficult problem in the current clinical anti-infective treatment. We performed a retrospective analysis of prevalence and treatment for CRE infections patients. METHODS This study was conducted in three tertiary care hospitals from January 1, 2010 to December 30, 2016. Baseline data, trea...

We characterized 97 non-O157 Shiga toxin (stx)-producing Escherichia coli strains isolated from human patients during 2000-2009 from the national reference laboratory in Switzerland. These strains belonged to 40 O:H serotypes; 4 serotypes (O26:H11/H-, O103:H2, O121:H19, and O145:H28/H-) accounted for 46.4% of the strains. Nonbloody diarrhea was reported by 23.2% of the patients, bloody diarrhea...

______________ 1 The work was partially supported by the Russian Foundation for Basic Research (Project No. 08-08-00155-a). Abstract – Switching of H11 mode in a circular waveguide of the interference switch improves parameters of high power microwave compressors. The switch can stand more than 3.5 ⋅ 10 6 operations and provides the rms 2% of output pulse amplitude deviation at the repetitions ...

Live animal markets (LAMs) are an essential source of food and trade in Latin American countries; however, they can also serve as 'hotbeds' for the emergence and potential spillover of avian influenza viruses (AIV). Despite extensive knowledge of AIV in Asian LAMs, little is known about the prevalence South American LAMs. To fill this gap in knowledge, active surveillance was carried out at the...